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Construction of green fluorescent protein reporter genes for genotoxicity test (SOS/ umu-test) and improvement of mutagen-sensitivity

Green fluorescent protein (GFP) reporter genes for the bacterial umu-test were constructed. Utilization of tandem, lacUV5 and chimeric trp/umu promoters, and coexpression of the Escherichia coli recA5327 mutant enhanced the GFP expression level fourteen-fold over that of the system with only the umu...

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Published in:Journal of bioscience and bioengineering 2001, Vol.92 (3), p.301-304
Main Authors: Arai, Ryoichi, Makita, Yukimasa, Oda, Yoshimitsu, Nagamune, Teruyuki
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Language:English
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cited_by cdi_FETCH-LOGICAL-c478t-5d813c8b3116bd2566229b4203a64d311dc08362da61e32ffb8dba40e6b5aa3
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container_title Journal of bioscience and bioengineering
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creator Arai, Ryoichi
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description Green fluorescent protein (GFP) reporter genes for the bacterial umu-test were constructed. Utilization of tandem, lacUV5 and chimeric trp/umu promoters, and coexpression of the Escherichia coli recA5327 mutant enhanced the GFP expression level fourteen-fold over that of the system with only the umu promoter, thereby improving the sensitivity of the umu-test.
doi_str_mv 10.1016/S1389-1723(01)80267-X
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identifier ISSN: 1389-1723
ispartof Journal of bioscience and bioengineering, 2001, Vol.92 (3), p.301-304
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source ScienceDirect Freedom Collection 2022-2024
subjects Biological and medical sciences
chimeric promoter
FLUORESCENCE
General aspects. Methods
GENES
GENOTOXICITY
green fluorescent protein (GFP)
Medical sciences
MUTAGENS
PROTEINS
recA mutant
tandem promoter
Toxicology
umu-test
title Construction of green fluorescent protein reporter genes for genotoxicity test (SOS/ umu-test) and improvement of mutagen-sensitivity
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