Novel male-specific molecular markers (MADC5, MADC6) in hemp

Decamer RAPD primers were tested on dioecious and monoecious hemp cultivars to identify sex-specific molecular markers. Two primers (OPD05 and UBC354) generated specific bands in male plants. These two DNA fragments were isolated, cloned and sequenced. Both markers proved to be unique, since no sequ...

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Published in:Euphytica 2002-01, Vol.127 (2), p.209-218
Main Authors: TÖRJEK, Otto, BUCHERNA, Nandor, KISS, Erzsébet, HOMOKI, Hajnalka, FINTA-KORPELOVA, Zsuzsanna, BOCSA, Ivan, NAGY, Istvan, HESZKY, Laszlo E
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Cannabis
Classical and quantitative genetics. Population genetics. Molecular genetics
Cultivars
Deoxyribonucleic acid
DNA
Fundamental and applied biological sciences. Psychology
Generalities. Genetics. Plant material
Genetics
Genetics and breeding of economic plants
Hemp
Homology
Males
Markers
Molecular genetics
Nucleotide sequence
Phenotypes
Plant populations
Population studies
Sex
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container_end_page 218
container_issue 2
container_start_page 209
container_title Euphytica
container_volume 127
creator TÖRJEK, Otto
BUCHERNA, Nandor
KISS, Erzsébet
HOMOKI, Hajnalka
FINTA-KORPELOVA, Zsuzsanna
BOCSA, Ivan
NAGY, Istvan
HESZKY, Laszlo E
description Decamer RAPD primers were tested on dioecious and monoecious hemp cultivars to identify sex-specific molecular markers. Two primers (OPD05 and UBC354) generated specific bands in male plants. These two DNA fragments were isolated, cloned and sequenced. Both markers proved to be unique, since no sequence with significant homology to OPD05961 and UBC354151 markers were found in databases. These markers were named MADC3 (OPD05961) and MADC4 (UBC354151) (Male-Associated DNA from Cannabis sativa). The markers were converted into sequence-characterized amplified region (SCAR) markers. The SCAR markers correlated with the sex of the segregating F2 population and proved the tight linkage to the male phenotype. Results of F2 plant population analysis suggest these markers are to be linked to the Y chromosome.
doi_str_mv 10.1023/A:1020204729122
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source Springer Nature
subjects Agronomy. Soil science and plant productions
Biological and medical sciences
Cannabis
Classical and quantitative genetics. Population genetics. Molecular genetics
Cultivars
Deoxyribonucleic acid
DNA
Fundamental and applied biological sciences. Psychology
Generalities. Genetics. Plant material
Genetics
Genetics and breeding of economic plants
Hemp
Homology
Males
Markers
Molecular genetics
Nucleotide sequence
Phenotypes
Plant populations
Population studies
Sex
title Novel male-specific molecular markers (MADC5, MADC6) in hemp
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