Congo red dye affects survival and reproduction in the cladoceran Ceriodaphnia dubia. Effects of direct and dietary exposure

• Published in: Ecotoxicology (London) 2016-12, Vol.25 (10), p.1832-1840
• Main Authors: Hernández-Zamora, Miriam, Martínez-Jerónimo, Fernando, Cristiani-Urbina, Eliseo, Cañizares-Villanueva, Rosa Olivia
• Format: Article
• Language: English
• Subjects: Algae; Analysis; Animals; Aquatic ecosystems; Aquatic environment; Azo dyes; Ceriodaphnia dubia; Cladocera; Cladocera - drug effects; Cladocera - physiology; Congo Red - toxicity; Dyes; Earth and Environmental Science; Ecology; Ecotoxicology; Environment; Environmental Management; Fertility; Health aspects; Health risks; Lethal Dose 50; Pulp & paper industry; Reproduction; Survival; Toxicity Tests; Waste Water; Wastewater; Wastewater discharges; Water Pollutants, Chemical - toxicity
• ISSN: 0963-9292; 1573-3017
• DOI: 10.1007/s10646-016-1731-x

Nearly 7 00000 tons of dyes are produced annually throughout the world. Azo dyes are widely used in the textile and paper industries due to their low cost and ease of application. Their extensive use results in large volumes of wastewater being discharged into aquatic ecosystems. Large volume discharges constitute a health risk since many of these dyes, such as Congo Red, are elaborated with benzidine, a known carcinogenic compound. Information regarding dye toxicity in aquatic ecosystems is limited. Therefore, the aim of the present study was to evaluate the effect of Congo Red on survival and reproduction of Ceriodaphnia dubia . We determined the 48 h median lethal concentration (LC 50 ) and evaluated the effects of sublethal concentrations in subchronic exposures by using as food either fresh algae or algae previously exposed to the dye. LC 50 was 13.58 mg L −1 . In subchronic assays, survival was reduced to 80 and 55 %, and fertility to 40 and 70 %, as compared to the control, in C. dubia fed with intoxicated cells or with the mix of intoxicated + fresh algae, respectively, so the quantity and type of food had a significant effect. We determined that Congo Red is highly toxic to C. dubia since it inhibits survival and fertility in concentrations exceeding 3 mg L −1 . Our results show that this dye produces negative effects at very low concentrations. Furthermore, our findings warn of the risk associated with discharging dyes into aquatic environments. Lastly, the results emphasize the need to regulate the discharge of effluents containing azo dyes.