Transcriptomic response of Drosophila melanogaster pupae developed in hypergravity

Altered gravity can perturb normal development and induce corresponding changes in gene expression. Understanding this relationship between the physical environment and a biological response is important for NASA's space travel goals. We use RNA-Seq and qRT-PCR techniques to profile changes in...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2016-10, Vol.108 (3-4), p.158-167
Main Authors: Hateley, Shannon, Hosamani, Ravikumar, Bhardwaj, Shilpa R., Pachter, Lior, Bhattacharya, Sharmila
Format: Article
Language:English
Subjects:
Animals
Drosophila melanogaster
Drosophila melanogaster - embryology
Drosophila melanogaster - genetics
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Gene Expression Regulation, Developmental
Hypergravity
Metamorphosis
Pupa - genetics
Pupae
RNA-Seq
Transcriptome
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Summary:Altered gravity can perturb normal development and induce corresponding changes in gene expression. Understanding this relationship between the physical environment and a biological response is important for NASA's space travel goals. We use RNA-Seq and qRT-PCR techniques to profile changes in early Drosophila melanogaster pupae exposed to chronic hypergravity (3g, or three times Earth's gravity). During the pupal stage, D. melanogaster rely upon gravitational cues for proper development. Assessing gene expression changes in the pupae under altered gravity conditions helps highlight gravity-dependent genetic pathways. A robust transcriptional response was observed in hypergravity-treated pupae compared to controls, with 1513 genes showing a significant (q
ISSN:0888-7543
1089-8646
DOI:10.1016/j.ygeno.2016.09.002