Hematological responses of tambaqui Colossoma macropomum (Serrassalmidae) fed with diets supplemented with essential oil from Mentha piperita (Lamiaceae) and challenged with Aeromonas hydrophila

• Published in: Acta amazonica 2016-03, Vol.46 (1)
• Main Authors: Ribeiro, Suzana Cardoso, Castelo, Antonielson Silva, da Silva, Bruna Marjara Picanco, Cunha, Andreza da Silva, Proietti Junior, Aldo Aparecido Proietti, Oba-Yoshioka, Eliane Tie
• Format: Article
• Language: Portuguese
• Subjects: Aeromonas hydrophila; Colossoma macropomum; Lamiaceae; Mentha piperita
• ISSN: 0044-5967; 1809-4392
• DOI: 10.1590/1809-4392201501284

In fish farmings, diseases can be reduced by using immunostimulants. The aim of this study was to evaluate the immunostimulant potential of Mentha piperita in tambaqui fed with 0, 0.5, 1.0 and 1.5% of oil per kg of commercial fish feed. The fish were inoculated with Aeromonas hydrophila to challenge them. Hematological and biochemical parameters were determined after 30 days of feeding and seven days after the challenge. There was no mortality and M. piperita oil did not influence fish production parameters. However, blood hemoglobin concentration (Hb) increased in the fish fed with 0.5 and 1.5% of oil per kg of diet; albumin increased in those fed with 1.0%; cholesterol increased in all groups with oil; and triglycerides increased in those fed with 0.5%. After the bacterial challenge, the fish showed decreases in Hb when fed with diet enriched with 1.5% oil per kg of diet, in mean corpuscular volume with 1.0% and in mean corpuscular hemoglobin concentration with 0 and 1.5%. Protein levels increased in groups with 0 and 1.5% of oil and albumin when fed with 0 and 1.0%; cholesterol levels increased in the control group; and high levels of triglycerides were observed in the groups with 0, 0.5 and 1.5%. Thus, M. piperita essential oil promoted hematological alterations in tambaqui and can be recommended in diets containing up to 1.0% per kg, because of the minimal physiological modifications caused. However, additional studies are necessary to obtain more information regarding to the physiological effects of this immunostimulant.Original Abstract: Em pisciculturas, doencas podem ser minimizadas com o uso de imunoestimulantes. O objetivo deste estudo foi avaliar o potencial imunoestimulante do oleo essencial de Mentha piperita na dieta de tambaqui com dietas contendo 0; 0,5; 1,0; 1,5% de inclusao do oleo por kg de racao comercial para peixes. Os tambaquis foram desafiados com a bacteria Aeromonas hydrophila. Hematologia e bioquimica dos tambaquis foram determinados apos 30 dias de alimentacao e sete dias do desafio. Nao houve mortalidade dos peixes durante o experimento, sendo que o oleo nao influenciou os parametros produtivos dos peixes. Entretanto, a concentracao de hemoglobina (Hb) aumentou em peixes alimentados com 0,5 e 1,5% de oleo por kg de dieta; a albumina aumentou em 1,0%; o colesterol aumentou em todos os grupos com oleo; e triglicerides em 0,5% por kg de dieta. Apos desafio bacteriano ocorreram diminuicoes da Hb nos peixes alimentados com 1,5% de