Microbiota induces expression of tumor necrosis factor in postnatal mouse skin

Tumor necrosis factor (TNF) is a pleiotropic cytokine that regulates many important processes in the body. TNF production in a physiological state supports the structure of lymphoid organs and determines the development of lymphoid cells in hematopoiesis. However, chronic TNF overexpression leads to...

Full description

Saved in:
Bibliographic Details
Published in:Biochemistry (Moscow) 2016-11, Vol.81 (11), p.1303-1308
Main Authors: Yuzhakova, D. V., Shirmanova, M. V., Bocharov, A. A., Astrakhantseva, I. V., Vasilenko, E. A., Gorshkova, E. N., Drutskaya, M. S., Zagaynova, E. V., Nedospasov, S. A., Kruglov, A. A.
Format: Article
Language:English
Subjects:
Animals
Antibiotics
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Fluorescence
Gene Expression Regulation - immunology
Immune system
Life Sciences
Mice
Mice, Transgenic
Microbiology
Microbiota - immunology
Molecular and Cellular Mechanisms of Inflammation (Special Issue) Guest Editors S. A. Nedospasov and D. V. Kuprash
Protein expression
Rodents
Scientific imaging
Skin
Skin - immunology
Skin - metabolism
Skin - microbiology
Tissue analysis
Tumor Necrosis Factor-alpha - biosynthesis
Tumor Necrosis Factor-alpha - immunology
Tumor necrosis factor-TNF
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Tumor necrosis factor (TNF) is a pleiotropic cytokine that regulates many important processes in the body. TNF production in a physiological state supports the structure of lymphoid organs and determines the development of lymphoid cells in hematopoiesis. However, chronic TNF overexpression leads to the development of various autoimmune disorders. Sites of TNF production in the naive state remain unclear due to the lack of in vivo models. In the present study, we used TNF-2A-Kat reporter mice to monitor the expression of TNF in different tissues. Comparative analysis of tissue fluorescence in TNF-2A-Kat reporter mice and wild type mice revealed constitutive expression of TNF in the skin of naive adult mice. In the skin of TNF-2A-Kat reporter mouse embryos, no statistically significant differences in the expression of TNF compared to wild type animals were observed. Furthermore, we established that local depletion of microflora with topical antibiotics leads to a reduction in the fluorescence signal. Thus, we assume that the skin microflora is responsible for the expression of TNF in the skin of mice.
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297916110080