Comparison of in vitro bioactivity of chicken prolactin and mammalian lactogenic hormones

Highlights • Large scale preparation of recombinant chicken prolactin (chPRL). • Evaluation of prolactins and other lactogenic hormones activity using heterologous and homologous bioassays. • The homologous assay are valid for predicting the activity of species-specific lactogenic hormones in vivo a...

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Published in:General and comparative endocrinology 2017-01, Vol.240, p.27-34
Main Authors: Ocłoń, Ewa, Leśniak-Walentyn, Agnieszka, Solomon, Gili, Shpilman, Michal, Hrabia, Anna, Gertler, Arieh
Format: Article
Language:English
Subjects:
Animals
Bioactivity
Chicken
Chickens - metabolism
Endocrinology & Metabolism
Escherichia coli
Humans
Mammals
Prolactin
Prolactin - metabolism
Rabbits
Recombinant
Sheep
Species Specificity
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cited_by cdi_FETCH-LOGICAL-c447t-33ccef5f9c42843ba4016650e40e61cf56eed6124fc4f20a64d444b745b1e1fb3
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container_title General and comparative endocrinology
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creator Ocłoń, Ewa
Leśniak-Walentyn, Agnieszka
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Hrabia, Anna
Gertler, Arieh
description Highlights • Large scale preparation of recombinant chicken prolactin (chPRL). • Evaluation of prolactins and other lactogenic hormones activity using heterologous and homologous bioassays. • The homologous assay are valid for predicting the activity of species-specific lactogenic hormones in vivo activity, whereas heterologous bioassays might be misleading.
doi_str_mv 10.1016/j.ygcen.2016.09.008
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subjects Animals
Bioactivity
Chicken
Chickens - metabolism
Endocrinology & Metabolism
Escherichia coli
Humans
Mammals
Prolactin
Prolactin - metabolism
Rabbits
Recombinant
Sheep
Species Specificity
title Comparison of in vitro bioactivity of chicken prolactin and mammalian lactogenic hormones
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