Novel Functional Genomics Approaches: A Promising Future in the Combat Against Plant Viruses

Advances in functional genomics and genome editing approaches have provided new opportunities and potential to accelerate plant virus control efforts through modification of host and viral genomes in a precise and predictable manner. Here, we discuss application of RNA-based technologies, including...

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Bibliographic Details
Published in:Phytopathology 2016-10, Vol.106 (10), p.1231-1239
Main Authors: Fondong, Vincent N, Nagalakshmi, Ugrappa, Dinesh-Kumar, Savithramma P
Format: Article
Language:English
Subjects:
Breeding
CRISPR-Associated Proteins - genetics
Disease Resistance - genetics
Genes, Recessive - genetics
Genome, Plant - genetics
Genomics
MicroRNAs - genetics
Plant Diseases - immunology
Plant Diseases - prevention & control
Plant Diseases - virology
Plant Viruses - physiology
Plants - genetics
Plants - immunology
Plants - virology
Reverse Genetics
RNA, Plant - genetics
RNA, Small Interfering - genetics
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Summary:Advances in functional genomics and genome editing approaches have provided new opportunities and potential to accelerate plant virus control efforts through modification of host and viral genomes in a precise and predictable manner. Here, we discuss application of RNA-based technologies, including artificial micro RNA, transacting small interfering RNA, and Cas9 (clustered regularly interspaced short palindromic repeat-associated protein 9), which are currently being successfully deployed in generating virus-resistant plants. We further discuss the reverse genetics approach, targeting induced local lesions in genomes (TILLING) and its variant, known as EcoTILLING, that are used in the identification of plant virus recessive resistance gene alleles. In addition to describing specific applications of these technologies in plant virus control, this review discusses their advantages and limitations.
ISSN:0031-949X
1943-7684
DOI:10.1094/PHYTO-03-16-0145-FI