Two-stage cultivation of Nannochloropsis oculata for lipid production using reversible alkaline flocculation

[Display omitted] •Alkaline flocculation could replace centrifugation in two-stage cultivation.•Total lipid content increased >3-fold during the nitrogen-depleted cultivation.•Biomass was enriched in C16 fatty acids methyl esters after two-stage cultivation. Two-stage cultivation for microalgae b...

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Bibliographic Details
Published in:Bioresource technology 2017-02, Vol.226, p.18-23
Main Authors: Aléman-Nava, Gibran Sidney, Muylaert, Koenraad, Cuellar Bermudez, Sara Paulina, Depraetere, Orily, Rittmann, Bruce, Parra-Saldívar, Roberto, Vandamme, Dries
Format: Article
Language:English
Subjects:
Algae
Biomass
Biotechnology - methods
Centrifugation
Fatty Acids - chemistry
Fatty Acids - metabolism
Flocculation
Lipids
Lipids - biosynthesis
Lipids - chemistry
Microalgae - growth & development
Microalgae - metabolism
Nutrient starvation
Photosystem II Protein Complex - metabolism
Separation
Stramenopiles - growth & development
Stramenopiles - metabolism
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Summary:[Display omitted] •Alkaline flocculation could replace centrifugation in two-stage cultivation.•Total lipid content increased >3-fold during the nitrogen-depleted cultivation.•Biomass was enriched in C16 fatty acids methyl esters after two-stage cultivation. Two-stage cultivation for microalgae biomass is a promising strategy to boost lipid accumulation and productivity. Most of the currently described processes use energy-intensive centrifugation for cell separation after the first cultivation stage. This laboratory study evaluated alkaline flocculation as low-cost alternative separation method to harvest Nannochloropsis oculata prior to cultivation in the second nutrient-depleted cultivation stage. Biomass concentration over time and the maximum quantum yield of photosystem II expressed as Fv:Fm ratio showed identical patterns for both harvesting methods in both stages. The composition of total lipids, carbohydrates, and protein was similar for biomass harvested via alkaline flocculation or centrifugation. Likewise, both harvest methods yielded the same increase in total lipid content, to 40% within the first 2days of the nutrient-depleted stage, with an enrichment in C16 fatty acid methyl esters. Centrifugation can therefore be replaced with alkaline flocculation to harvest Nannochloropsis oculata after the first cultivation stage.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2016.11.121