Targeted suppression of miRNA-21 inhibit K562 cells growth through PTEN-PI3K/AKT signaling pathway

To investigate the K562 cells biological function and related molecular changes in PTEN-PI3K/AKT signaling pathway of leukemia K562 cells by inhibiting the miRNA-21 expression to explore its pathogenesis of leukemia. The chemical synthetic miRNA-21 inhibitor was transfered into K562 cells by electro...

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Bibliographic Details
Published in:Zhōnghuá xuèyèxué zázhì 2016-11, Vol.37 (11), p.982
Main Authors: Liu, M H, Yang, L, Liu, X J, Nie, Z Y, Luo, J M
Format: Article
Language:Chinese
Subjects:
Apoptosis
Cell Cycle
Cell Proliferation
Down-Regulation
Humans
K562 Cells
Leukemia
MicroRNAs - physiology
Phosphatidylinositol 3-Kinases - metabolism
Proto-Oncogene Proteins c-akt - metabolism
PTEN Phosphohydrolase
Signal Transduction
Up-Regulation
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Summary:To investigate the K562 cells biological function and related molecular changes in PTEN-PI3K/AKT signaling pathway of leukemia K562 cells by inhibiting the miRNA-21 expression to explore its pathogenesis of leukemia. The chemical synthetic miRNA-21 inhibitor was transfered into K562 cells by electrotransfection. RT-PCR was used to detect the miRNA-21 expression changes. Cell proliferation and apoptosis were determined by using MTT and flow cytometry. Western-blot were used to detect the protein expression changes of PTEN, PI3K and p-AKT respectively. The relative expression of miRNA-21 in experimental group was (8.070 ± 5.138)% at 24 hours, which was lower than control groups (
ISSN:0253-2727
DOI:10.3760/cma.j.issn.0253-2727.2016.11.011