Size-dependent cellular uptake of exosomes

Abstract The ability of exosomes to elicit specific cellular responses suggests that they may be increasingly used as therapeutics. Their vesicular nature makes them suitable as potential nanocarriers for drugs or nucleic acids delivery. Here we address the question whether the method of preparation...

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Bibliographic Details
Published in:Nanomedicine 2017-04, Vol.13 (3), p.1011-1020
Main Authors: Caponnetto, Federica, Manini, Ivana, Skrap, Miran, Palmai-Pallag, Timea, Di Loreto, Carla, Beltrami, Antonio Paolo, Cesselli, Daniela, Ferrari, Enrico
Format: Article
Language:English
Subjects:
AFM
Cell Fractionation
Cell Line, Tumor
Cell Movement
Cell Proliferation
Cellular uptake
Chemical Precipitation
Exosomes
Exosomes - metabolism
Exosomes - pathology
Exosomes - ultrastructure
Flow Cytometry
GASC
Glioma
Glioma - metabolism
Glioma - pathology
Humans
Internal Medicine
Tumor Cells, Cultured
Ultracentrifugation
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Summary:Abstract The ability of exosomes to elicit specific cellular responses suggests that they may be increasingly used as therapeutics. Their vesicular nature makes them suitable as potential nanocarriers for drugs or nucleic acids delivery. Here we address the question whether the method of preparation of enriched exosomal fractions can affect their uptake by cells and their ability to trigger a response. We compared ultracentrifugation and polymer-based precipitation methods on supernatants of glioma-associated stem cells isolated from a high-grade glioma patient. We determined particle size distributions after purification and their correlation with uptake, proliferation and migration in glioblastoma cell cultures. Our findings indicate that polymer-based precipitation leads to smaller particle size distributions, faster uptake by target cells and increased cellular motility. The different effect that isolation method-dependent populations of particles have on cell motility suggests their size distribution could also profoundly affect exosome therapeutic potential.
ISSN:1549-9634
1549-9642
DOI:10.1016/j.nano.2016.12.009