Survey of retinal ganglion cell morphology in marmoset

• Published in: Journal of comparative neurology (1911) 2019-01, Vol.527 (1), p.236-258
• Main Authors: Masri, Rania A., Percival, Kumiko A., Koizumi, Amane, Martin, Paul R., Grünert, Ulrike
• Format: Article
• Language: English
• Subjects: Amacrine cells; Animals; Callithrix - anatomy & histology; Cytology; Eye; Female; Gene transfer; Green fluorescent protein; Male; Melanopsin; midget cells; Morphology; parasol cells; Postsynaptic density; primate; Retina; Retinal ganglion cells; Retinal Ganglion Cells - classification; Retinal Ganglion Cells - cytology; RRID: AB_10000340; RRID: AB_10000347; RRID: AB_2079751; RRID: AB_231413; RRID: AB_399431; wide‐field ganglion cells
• ISSN: 0021-9967; 1096-9861
• DOI: 10.1002/cne.24157

In primate retina, the midget, parasol, and small bistratified cell populations form the large majority of ganglion cells. In addition, there is a variety of low‐density wide‐field ganglion cell types that are less well characterized. Here we studied retinal ganglion cells in the common marmoset, Callithrix jacchus, using particle‐mediated gene transfer. Ganglion cells were transfected with an expression plasmid for the postsynaptic density 95–green fluorescent protein. The retinas were processed with established immunohistochemical markers for bipolar and/or amacrine cells to determine ganglion cell dendritic stratification. In total over 500 ganglion cells were classified based on their dendritic field size, morphology, and stratification in the inner plexiform layer. Over 17 types were distinguished, including midget, parasol, broad thorny, small bistratified, large bistratified, recursive bistratified, recursive monostratified, narrow thorny, smooth monostratified, large sparse, giant sparse (melanopsin) ganglion cells, and a group that may contain several as yet uncharacterized types. Assuming each characterized type forms a hexagonal mosaic, the midget and parasol cells account for over 80% of all ganglion cells in the central retina but only ∼50% of cells in the peripheral (>2 mm) retina. We conclude that the fovea is dominated by midget and parasol cells, but outside the fovea the ganglion cell diversity in marmoset is likely as great as that reported for nonprimate retinas. Taken together, the ganglion cell types in marmoset retina resemble those described previously in macaque retina with respect to morphology, stratification, and change in proportion across the retina. Over 17 types of ganglion cell were distinguished in marmoset retina. The study shows that the fovea is dominated by midget and parasol cells, but outside the fovea wide‐field ganglion cell types make up a significant proportion of the total ganglion cell population. Scale bar = 100 μm.