The EB66® cell line as a valuable cell substrate for MVA-based vaccines production

Highlights • EB66® cell line is a valuable cell substrate for production of MVA-based vaccine. • Pilot-scale production process was established that yields high titres. • Pilot-scale purification process has been developed. • Virus attenuation and transgene expression are maintained in EB66® cells....

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Bibliographic Details
Published in:Vaccine 2016-11, Vol.34 (48), p.5878-5885
Main Authors: Léon, Arnaud, David, Anne-Laure, Madeline, Brice, Guianvarc’h, Laurence, Dureau, Elodie, Champion-Arnaud, Patrick, Hebben, Matthias, Huss, Thierry, Chatrenet, Benoît, Schwamborn, Klaus
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Antigens, Viral
Aquatic birds
Cell culture
Cell Line
Deoxyribonucleic acid
DNA
Ducks
EB66® cells
Embryo, Nonmammalian - cytology
Fibroblasts
Genetic engineering
Genomes
Glucose
Good Manufacturing Practice
Humans
Industrialization
Infections
Manufacturing
Manufacturing process
Modified vaccinia virus Ankara
Mutation
Serial Passage
Substrates
Transgenes
Vaccine production
Vaccines
Vaccines, Attenuated
Vaccinia virus - genetics
Vaccinia virus - immunology
Vaccinia virus - pathogenicity
Vaccinia virus - physiology
Viral Vaccines
Virus Cultivation
Virus Replication
Viruses
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Summary:Highlights • EB66® cell line is a valuable cell substrate for production of MVA-based vaccine. • Pilot-scale production process was established that yields high titres. • Pilot-scale purification process has been developed. • Virus attenuation and transgene expression are maintained in EB66® cells.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2016.10.043