Plasmonic Nanosensors for the Determination of Drug Effectiveness on Membrane Receptors

We demonstrate the potential of the NanoSPR (nanoscale surface plasmon resonance sensors) method as a simple and cheap tool for the quantitative study of membrane protein–protein interactions. We use NanoSPR to determine the effectiveness of two potential drug candidates that inhibit the protein com...

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Bibliographic Details
Published in:ACS applied materials & interfaces 2017-01, Vol.9 (1), p.218-223
Main Authors: Ahijado-Guzmán, Rubén, Menten, Julia, Prasad, Janak, Lambertz, Christina, Rivas, Germán, Sönnichsen, Carsten
Format: Article
Language:English
Subjects:
Bacterial Proteins
Carrier Proteins
Cell Cycle Proteins
Cytoskeletal Proteins
Escherichia coli
Escherichia coli Proteins
Nanostructures
Protein Binding
Surface Plasmon Resonance
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Summary:We demonstrate the potential of the NanoSPR (nanoscale surface plasmon resonance sensors) method as a simple and cheap tool for the quantitative study of membrane protein–protein interactions. We use NanoSPR to determine the effectiveness of two potential drug candidates that inhibit the protein complex formation between FtsA and ZipA at initial stages of bacterial division. As the NanoSPR method relies on individual gold nanorods as sensing elements, there is no need for fluorescent labels or organic cosolvents, and it provides intrinsically high statistics. NanoSPR could become a powerful tool in drug development, drug delivery, and membrane studies.
ISSN:1944-8244
1944-8252
DOI:10.1021/acsami.6b14013