IncN ST7 epidemic plasmid carrying blaIMP-4 in Enterobacteriaceae isolates with epidemiological links to multiple geographical areas in China

To characterize bla -carrying plasmids originating from inpatients in Hong Kong. Sixteen bla -carrying plasmids identified among Enterobacteriaceae (nine Escherichia coli, four Klebsiella pneumoniae, two Citrobacter freundii and one Enterobacter cloacae) recovered from 15 patients were characterized...

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Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 2017-01, Vol.72 (1), p.99-103
Main Authors: Wang, Ya, Lo, Wai-U, Lai, Raymond Wai-Man, Tse, Cindy Wing-Sze, Lee, Rodney Allan, Luk, Wei-Kwang, Cheng, Vincent Chi-Chung, Que, Tak-Lun, Chow, Kin-Hung, Ho, Pak-Leung
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Cross Infection - epidemiology
Cross Infection - microbiology
Enterobacteriaceae - enzymology
Enterobacteriaceae - genetics
Enterobacteriaceae - isolation & purification
Enterobacteriaceae Infections - epidemiology
Enterobacteriaceae Infections - microbiology
Female
Gene Transfer, Horizontal
Hong Kong - epidemiology
Humans
Infant
Male
Middle Aged
Plasmids - analysis
Plasmids - classification
Retrospective Studies
Sequence Alignment
Sequence Analysis, DNA
Topography, Medical
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Summary:To characterize bla -carrying plasmids originating from inpatients in Hong Kong. Sixteen bla -carrying plasmids identified among Enterobacteriaceae (nine Escherichia coli, four Klebsiella pneumoniae, two Citrobacter freundii and one Enterobacter cloacae) recovered from 15 patients were characterized. The isolates, collected during January 2010 to December 2013, were retrospectively investigated by plasmid sequencing, molecular and fitness studies. The bla -carrying plasmids belonged to the IncN ST7 lineage (∼50 kb). Twelve of the 16 plasmids were epidemiologically linked to seven different regions in China. Alignment of the complete plasmid sequences showed identical plasmid backbones and two highly similar resistance regions, each carrying one of two resistance genes (bla and qnrS1). The bla was detected in a class 1 integron (containing bla and intron Kl.pn.13) that is part of an IS6100-IS26 transposon-like structure. The nine E. coli carrying the epidemic plasmid belonged to multiple multilocus STs (six ST542, one ST131, one ST657 and one ST3177). Fitness assays performed on E. coli J53 recipients showed that the presence of the epidemic plasmid did not have a significant biological cost. This study identified a bla -carrying IncN ST7 plasmid disseminated among multiple enterobacterial species originating from patients with epidemiological links to different regions in China.
ISSN:1460-2091