Expression of Wif-1 and β-catenin in the Wnt pathway in childhood acute lympho-blastic leukemia

• Published in: Zhongguo dang dai er ke za zhi 2016-09, Vol.18 (9), p.835-839
• Main Authors: Gao, Ji-Zhao, Zhao, Ji-Ou, Tan, Ying
• Format: Article
• Language: Chinese
• Subjects: Adaptor Proteins, Signal Transducing - genetics; Adaptor Proteins, Signal Transducing - physiology; Adolescent; beta Catenin - genetics; beta Catenin - physiology; Child; Child, Preschool; Female; Humans; Infant; Male; Precursor Cell Lymphoblastic Leukemia-Lymphoma - etiology; Precursor Cell Lymphoblastic Leukemia-Lymphoma - physiopathology; Repressor Proteins - genetics; Repressor Proteins - physiology; RNA, Messenger - analysis; Wnt Signaling Pathway - physiology
• ISSN: 1008-8830
• DOI: 10.7499/j.issn.1008-8830.2016.09.010

To investigate the expression and possible roles of Wnt inhibitory factor-1 (Wif-1) and β-catenin in the Wnt pathway in childhood acute lymphoblastic leukemia (ALL). The clinical data of 35 children who had newly-diagnosed ALL and achieved complete remission on day 33 of remission induction therapy were retrospectively reviewed. The children before treatment were considered as the incipient group, and those who achieved complete remission on day 33 were considered as the remission group. Fifteen children with non-malignant hematologic diseases were enrolled as the control group. RT-PCR was used to measure the mRNA expression of Wif-1 and β-catenin. ELISA was used to measure the protein expression of Wif-1. Compared with the control and remission groups, the incipient group had significantly lower mRNA and protein expression of Wif-1 and significantly higher mRNA expression of β-catenin (P