Gastrodia elata attenuates inflammatory response by inhibiting the NF-κB pathway in rheumatoid arthritis fibroblast-like synoviocytes

• Published in: Biomedicine & pharmacotherapy 2017-01, Vol.85, p.177-181
• Main Authors: Li, Yu, Wang, Li-min, Xu, Jian-zhong, Tian, Ke, Gu, Chen-xi, Li, Zhi-fu
• Format: Article
• Language: English
• Subjects: Anti-Inflammatory Agents - isolation & purification; Anti-Inflammatory Agents - pharmacology; Antirheumatic Agents - isolation & purification; Antirheumatic Agents - pharmacology; Arthritis, Rheumatoid - drug therapy; Arthritis, Rheumatoid - genetics; Arthritis, Rheumatoid - metabolism; Arthritis, Rheumatoid - pathology; Dose-Response Relationship, Drug; Fibroblasts - drug effects; Fibroblasts - metabolism; Fibroblasts - pathology; Gastrodia - chemistry; Gastrodia elata; Humans; Inflammation; Inflammation Mediators - metabolism; Interleukin-6 - metabolism; Interleukin-8 - metabolism; Internal Medicine; Matrix Metalloproteinase 13 - genetics; Matrix Metalloproteinase 13 - metabolism; Matrix Metalloproteinase 3 - genetics; Matrix Metalloproteinase 3 - metabolism; Medical Education; NF-kappa B - metabolism; NF-KappaB Inhibitor alpha - metabolism; Phosphorylation; Phytotherapy; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Plants, Medicinal; Proteolysis; Rheumatoid arthritis fibroblast-like synoviocytes; Signal Transduction - drug effects; Synovial Membrane - drug effects; Synovial Membrane - metabolism; Synovial Membrane - pathology; Time Factors; Transcription Factor RelA - metabolism; Tumor Necrosis Factor-alpha - pharmacology; Tumor necrosis factor-α
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2016.11.136

Abstract Gastrodia elata (GE), which belongs to the Orchidaceae family, was found to possess anti-inflammatory activity. However, the effect of GE on inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS) remains largely unknown. Thus, the aim of this study was to investigate the effects of GE on tumor necrosis factor-α (TNF-α)-induced inflammatory response in RA-FLS and the underlying molecular mechanism was also explored. Our results demonstrated that GE significantly attenuated TNF-α-induced IL-6 and IL-8 production in RA-FLS. GE also inhibited TNF-α-induced MMP-3 and MMP-13 expression in RA-FLS. Furthermore, pretreatment with GE significantly attenuated TNF-α-induced the expression of p-p65 and IκBα degradation in RA-FLS. In conclusion, this study demonstrated for the first time that GE attenuated inflammatory response by inhibiting the NF-κB pathway signaling in RA-FLS. Thus, GE might have a therapeutic potential towards the treatment of RA.