Development of a Rainbow Lateral Flow Immunoassay for the Simultaneous Detection of Four Mycotoxins

A multiplex lateral flow immunoassay (LFIA) for the determination of the mycotoxins deoxynivalenol, zearalenone, and T2/HT2-toxin in barley was developed with luminescent quantum dots (QDs) as label. The synthesized QDs were hydrophilized by two strategies, that is, coating with an amphiphilic polym...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2017-08, Vol.65 (33), p.7121-7130
Main Authors: Foubert, Astrid, Beloglazova, Natalia V, Gordienko, Anna, Tessier, Mickael D, Drijvers, Emile, Hens, Zeger, De Saeger, Sarah
Format: Article
Language:English
Subjects:
Hordeum - chemistry
Immunoassay - instrumentation
Immunoassay - methods
Limit of Detection
Mycotoxins - analysis
Quantum Dots
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Summary:A multiplex lateral flow immunoassay (LFIA) for the determination of the mycotoxins deoxynivalenol, zearalenone, and T2/HT2-toxin in barley was developed with luminescent quantum dots (QDs) as label. The synthesized QDs were hydrophilized by two strategies, that is, coating with an amphiphilic polymer or silica. The water-soluble QDs were compared with regard to their bioconjugation with monoclonal antibody (mAb) and were tested on a LFIA. Silica-coated QDs that contained epoxy groups were most promising. Therefore, green, orange, and red epoxy-functionalized silica-coated QDs were conjugated with anti-ZEN, anti-DON, and anti-T2 mAb, respectively. The LFIA was developed in accordance with the European Commission legal limits with cutoff limits of 1000, 80, and 80 μg/kg for deoxynivalenol, zearalenone, and T2/HT2-toxin, respectively. The LFIA gave a fast result (15 min) with a low false-negative rate (
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.6b04157