Characteristics of surface layer proteins from two new and native strains of Lactobacillus brevis

• Published in: International journal of biological macromolecules 2017-02, Vol.95, p.1004-1010
• Main Authors: Mobarak Qamsari, Elahe, Kasra Kermanshahi, Rouha, Erfan, Mohammad, Ghadam, Parinaz, Sardari, Soroush, Eslami, Neda
• Format: Article
• Language: English
• Subjects: Bacterial Proteins - biosynthesis; Bacterial Proteins - genetics; Bacterial Proteins - isolation & purification; Cell Wall - chemistry; Cell Wall - genetics; Cell Wall - metabolism; Cell Wall - ultrastructure; Electrophoresis, Polyacrylamide Gel; Freeze Drying; Gene Expression; Lactobacillus brevis; Lactobacillus brevis - chemistry; Lactobacillus brevis - genetics; Lactobacillus brevis - metabolism; Lactobacillus brevis - ultrastructure; Membrane Glycoproteins - biosynthesis; Membrane Glycoproteins - genetics; Membrane Glycoproteins - isolation & purification; Microtomy; Monodispersity; Protein Structure, Secondary; Secondary structure; Staining and Labeling; Surface layer proteins; Thermal behavior; Zeta potential
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2016.10.089

In this work, some important characteristics of surface layer (S-layer) proteins extracted from two new and native Lactobacillus strains, L.brevis KM3 and L.brevis KM7, were investigated. The presence of S-layer on the external surface of L.brevis KM3 was displayed by thin sectioning and negative staining. SDS-PAGE analysis were shown same dominant protein bands approximately around 48kDa for both S-layer proteins. Moreover, the S-layer reappeared when LiCl treated cells were allowed to grow again. Protein secondary structure and thermal behavior were evaluated by using circular dichroism (CD) and differential scanning calorimetry (DSC), respectively. Both S-layer proteins had high content of β-sheet and low amount of α-helix. The thermograms of lyophilized S-layer proteins of L.brevis KM3 and L.brevis KM7 showed one transition peak at 67.9°C and 59.14°C, respectively. To determine monodispersity of extracted S-layer proteins, dynamic light scattering (DLS) was used. The results indicated that the main population of S-layer molecules in two tested lactobacillus strains were composed of monomer with an expected diameter close to 10nm. Furthermore, Zeta potential measurements were showed positive potential for both S-layer proteins, as expected. Our results could be used as the basis for biotechnological applications of these two new S-layer proteins.