Interstitial photodynamic therapy and glioblastoma: Light fractionation in a preclinical model

Background Glioblastoma is a high‐grade cerebral tumor with local recurrence and poor outcome. Photodynamic therapy (PDT) is a localized treatment based on the light activation of a photosensitizer (PS) in the presence of oxygen, which results in the formation of cytotoxic species. The delivery of f...

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Bibliographic Details
Published in:Lasers in surgery and medicine 2017-07, Vol.49 (5), p.506-515
Main Authors: Leroy, Henri‐Arthur, Vermandel, Maximilien, Vignion‐Dewalle, Anne‐Sophie, Leroux, Bertrand, Maurage, Claude‐Alain, Duhamel, Alain, Mordon, Serge, Reyns, Nicolas
Format: Article
Language:English
Subjects:
5‐ALA
Aminolevulinic Acid - therapeutic use
Animals
Apoptosis
Brain
Brain cancer
Brain Neoplasms - drug therapy
Brain Neoplasms - pathology
Cytotoxicity
Disease Models, Animal
Dose Fractionation
Edema
Fractionation
Glioblastoma
Glioblastoma - drug therapy
Glioblastoma - pathology
immunohistology
Infiltration
Lasers
Lesions
Light
Metastases
Photochemotherapy
Photodynamic therapy
Photosensitizing Agents - therapeutic use
Putamen
Quality
Rats
Rats, Nude
Rodents
Tissues
U87
Vascularization
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Summary:Background Glioblastoma is a high‐grade cerebral tumor with local recurrence and poor outcome. Photodynamic therapy (PDT) is a localized treatment based on the light activation of a photosensitizer (PS) in the presence of oxygen, which results in the formation of cytotoxic species. The delivery of fractionated light may enhance treatment efficacy by reoxygenating tissues. Objective To evaluate the efficiency of two light‐fractionation schemes using immunohistological data. Materials and Methods Human U87 cells were grafted into the right putamen of 39 nude rats. After PS precursor intake (5‐ALA), an optic fiber was introduced into the tumor. The rats were randomly divided into three groups: without light, with light split into 2 fractions and with light split into 5 fractions. Treatment effects were assessed using brain immunohistology. Results Fractionated treatments induced intratumoral necrosis (P 
ISSN:0196-8092
1096-9101
DOI:10.1002/lsm.22620