Enrichment of serum biomarkers by magnetic metal-organic framework composites

Highly efficient extraction of peptides from serum is critical for finding serum biomarkers using mass spectrometry, which still remains a great challenge. Currently, a bottom-up proteomics approach has been applied to discover serum biomarkers. However, the approach was labor intensive, time and co...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2017-03, Vol.409 (7), p.1895-1904
Main Authors: Wei, Ji-Ping, Wang, Heng, Luo, Tao, Zhou, Zhi-Jiang, Huang, Yan-Feng, Qiao, Bin
Format: Article
Language:English
Subjects:
Absorption
Acids
Analytical Chemistry
Biochemistry
Biological markers
Biomarkers
Biomarkers - blood
Characterization and Evaluation of Materials
Chemical engineering
Chemical properties
Chemistry
Chemistry and Materials Science
Diabetes
Diabetes Mellitus, Type 2 - blood
Ferrosoferric Oxide - chemistry
Food Science
Humans
Iron compounds
Iron oxides
Laboratory Medicine
Magnetics
Mass spectrometry
Microscopy, Electron, Scanning
Monitoring/Environmental Analysis
Peptides
Proteins
Proteomics
Research Paper
Scientific imaging
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Summary:Highly efficient extraction of peptides from serum is critical for finding serum biomarkers using mass spectrometry, which still remains a great challenge. Currently, a bottom-up proteomics approach has been applied to discover serum biomarkers. However, the approach was labor intensive, time and cost consuming, and cannot meet the requirements for clinical application. In this work, Fe 3 O 4 /C@MIL-100 composites were synthesized to efficiently capture peptides from microwave-assisted formic acid digests of BSA and human serum prior to MALDI-TOF MS analysis. Fe 3 O 4 /C@MIL-100 composites exhibited size-selective adsorption performance, thus providing a rapid and convenient approach to enrich low-abundance peptides. Notably, the peptides’ mass fingerprinting of serum digestions between type 2 diabetes mellitus (T2DM) and healthy persons were distinguishable, which indicated the potential ability of this technique for T2DM diagnosis and rapid biomarker discovery. Graphical Abstract Efficient extraction and identification of serum biomarkers using Fe 3 O 4 /C@MIL-100 composites from acid hydrolysate
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-016-0136-2