Thioesterase overexpression in Nicotiana benthamiana leaf increases the fatty acid flux into triacylgycerol

Increasing the oil content of leafy biomass is emerging as a sustainable source of vegetable oil to meet global demand. Transient gene expression in leaf provides a reproducible platform to study the effect of transgenes on lipid biosynthesis. We first generated a transgenic Nicotiana benthamiana li...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 2017-01, Vol.591 (2), p.448-456
Main Authors: El Tahchy, Anna, Reynolds, Kyle B., Petrie, James R., Singh, Surinder P., Vanhercke, Thomas
Format: Article
Language:English
Subjects:
Acetates - metabolism
Arabidopsis Proteins - metabolism
diacylglycerol
Fatty Acids - metabolism
Gene Expression
leaf oil
lipid biosynthesis
Nicotiana - genetics
Nicotiana - metabolism
Nicotiana benthamiana
Plant Leaves - metabolism
Plant Proteins - metabolism
Plants, Genetically Modified
thioesterase
triacylglycerol
Triglycerides - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Increasing the oil content of leafy biomass is emerging as a sustainable source of vegetable oil to meet global demand. Transient gene expression in leaf provides a reproducible platform to study the effect of transgenes on lipid biosynthesis. We first generated a transgenic Nicotiana benthamiana line containing high levels of triacylglycerol in the leaf tissue (31.4% by dry weight) by stably expressing WRI1, DGAT1 and OLEOSIN. We then used this line as a platform to test the effect of three Arabidopsis thaliana thioesterases (FATA1, FATA2 and FATB). Further increases in leaf oil content were observed with biochemical and lipid assays revealing an increase in the export of fatty acids from the chloroplast and a modification in the oil profile.
ISSN:0014-5793
1873-3468
DOI:10.1002/1873-3468.12539