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Bactericidal laser ablation of carbon dots: An in vitro study on wild-type and antibiotic-resistant Staphylococcus aureus

Suspensions of Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA) were irradiated with a 808-nm diode laser in the presence of different concentrations of carbon dots (C-dots). The effects of laser irradiation and C-dots on the bactericidal activity were separately investig...

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Bibliographic Details
Published in:Journal of photochemistry and photobiology. B, Biology Biology, 2017-01, Vol.166, p.323-332
Main Authors: Sattarahmady, N., Rezaie-Yazdi, M., Tondro, G.H., Akbari, N.
Format: Article
Language:English
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Summary:Suspensions of Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA) were irradiated with a 808-nm diode laser in the presence of different concentrations of carbon dots (C-dots). The effects of laser irradiation and C-dots on the bactericidal activity were separately investigated. The results indicated that without laser irradiation, C-dots caused to a significant cease in the colony formation of both wild and methicillin-resistance types of S. aureus in a concentration-dependent manner. On the other hand, the bactericidal effect of C-dots was accelerated upon laser irradiation. Mechanistic studies revealed that formation of reactive oxygen species and protein leakage, which occurred in the presence of C-dots, were greatly accelerated by laser irradiation. Moreover, field emission scanning electron microscopy (FESEM) images showed morphological changes in the bacterial surfaces in the presence of C-dots upon laser irradiations, while almost no changes in the bacteria morphology were observed in the presence of C-dots alone. Outer membrane damage was a synergism result of laser irradiation and presence of C-dots. •C-dots were synthesized and characterized.•C-dots ceased colony formation of S. aureus.•The bactericidal effect of C-dots was accelerated by 808-nm light irradiation.•The mechanism of bactericidal effect of C-dots/light was explored.
ISSN:1011-1344
1873-2682
DOI:10.1016/j.jphotobiol.2016.12.006