Preparation of ionic liquid-mediated imprinted monolith for selective capture and purification of corilagin

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2017-01, Vol.1041-1042, p.98-103
• Main Authors: Wang, Chao, Li, Xiang-Jie, Yang, Jian, Zhao, Yong-Xin, Liu, Zhao-Sheng, Aisa, Haji Akber
• Format: Article
• Language: English
• Subjects: Chromatography, High Pressure Liquid; Corilagin; Dimethyl Sulfoxide - chemistry; Dimethylformamide - chemistry; Glucosides - analysis; Glucosides - isolation & purification; HPLC; Hydrolyzable Tannins - analysis; Hydrolyzable Tannins - isolation & purification; Imidazoles - chemistry; Ionic liquid; Ionic Liquids - chemistry; Molecular Imprinting - methods; Molecularly imprinted polymers; Monolith; Nitrogen; Particle Size; Porosity; Solid phase extraction; Solid Phase Extraction - methods
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2016.12.023

•Corilagin-imprinted polymers was made in ionic liquid-mediated porogen.•The imprinting effect and retention of the resulting MIP was studied.•The greatest imprinting factor was up to 9.•High purity of corilagin up to 98.0% from the extract of phyllanthus urinaria L. can be achieved. A method for solid-phase extraction (SPE) of corilagin from natural plant extracts based on molecularly imprinted polymers (MIPs) was developed. For the preparation of corilagin-MIP monoliths, 4-vinylpyridine was used as functional monomer, and ethylene glycol dimethacrylate was used as cross-linking monomer, using a mixture of 1-butyl-3-methylimidazoliumtetrafluoroborate (ionic liquid)-N,N-dimethylformamide-dimethyl sulfoxide as a porogen. A morphological characteristic of the corilagin imprinted monolith was further studied by scanning electron microscopy and nitrogen sorption method. The greatest imprinting factor of COR was up to 9. The MIPs were used as solid-phase extraction (SPE) sorbents for purification of COR and the mean recoveries of corilagin was 78.0% with COR purity of 98.0% from the crude extract of phyllanthus urinaria L. The resulting COR-imprinted polymer also displayed the good performance of fragment imprinting polymer for gallic acid with the mean recoveries of 94.0% and purity of 99.7%.