Lycopene pretreatment improves hepatotoxicity induced by acetaminophen in C57BL/6 mice

• Published in: Bioorganic & medicinal chemistry 2017-02, Vol.25 (3), p.1057-1065
• Main Authors: Bandeira, Ana Carla Balthar, da Silva, Rafaella Cecília, Rossoni, Joamyr Victor, Figueiredo, Vivian Paulino, Talvani, André, Cangussú, Silvia Dantas, Bezerra, Frank Silva, Costa, Daniela Caldeira
• Format: Article
• Language: English
• Subjects: Acetaminophen; Acetaminophen - administration & dosage; Animals; Antioxidants - administration & dosage; Antioxidants - chemistry; Antioxidants - pharmacology; C57BL/6 mice; Carotenoids - administration & dosage; Carotenoids - chemistry; Carotenoids - pharmacology; Chemical and Drug Induced Liver Injury - drug therapy; Chemical and Drug Induced Liver Injury - metabolism; Chemical and Drug Induced Liver Injury - pathology; Dose-Response Relationship, Drug; Inflammation; Liver - drug effects; Liver - metabolism; Liver - pathology; Lycopene; Male; Mice; Mice, Inbred C57BL; Molecular Structure; Oxidation-Reduction; Oxidative Stress - drug effects; Redox imbalance; Structure-Activity Relationship
• ISSN: 0968-0896; 1464-3391
• DOI: 10.1016/j.bmc.2016.12.018

[Display omitted] Acetaminophen (APAP) is an antipyretic and analgesic drug that, in high doses, leads to severe liver injury and potentially death. Oxidative stress is an important event in APAP overdose. Researchers are looking for natural antioxidants with the potential to mitigate the harmful effects of reactive oxygen species in different models. Lycopene has been widely studied for its antioxidant properties. The aim of this study was to evaluate the antioxidant potential of lycopene pretreatment in APAP-induced liver injury in C57BL/6 mice. C57BL/6 male mice were divided into the following groups: control (C); sunflower oil (CO); acetaminophen 500mg/kg (APAP); acetaminophen 500mg/kg+lycopene 10mg/kg (APAP+L10), and acetaminophen 500mg/kg+lycopene 100mg/kg (APAP+L100). Mice were pretreated with lycopene for 14 consecutive days prior to APAP overdose. Analyses of blood serum and livers were performed. Lycopene was able to improve redox imbalance, decrease thiobarbituric acid reactive species level, and increase CAT and GSH levels. In addition, it decreased the IL-1β expression and the activity of MMP-2. This study revealed that preventive lycopene consumption in C57BL/6 mice can attenuate the effects of APAP-induced liver injury. Furthermore, by improving the redox state, and thus indicating its potential antioxidant effect, lycopene was also shown to have an influence on inflammatory events.