Genetic diversity among Babesia rossi detected in naturally infected dogs in Abeokuta, Nigeria, based on 18S rRNA gene sequences

Adequate knowledge of the genetic diversity among Babesia species infecting dogs is necessary for a better understanding of the epidemiology and control of canine babesiosis. Hence, this study determined the genetic diversity among the Babesia rossi detected in dogs presented for routine examination...

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Bibliographic Details
Published in:Acta parasitologica 2017-03, Vol.62 (1), p.192-198
Main Authors: Takeet, Michael I., Oyewusi, Adeoye J., Abakpa, Simon A. V., Daramola, Olukayode O., Peters, Sunday O.
Format: Article
Language:English
Subjects:
18s rDNA
Abeokuta
Animal Systematics/Taxonomy/Biogeography
Animals
Babesia - genetics
Babesiosis - epidemiology
Babesiosis - parasitology
Base Sequence
Biomedical and Life Sciences
Biomedicine
Dog Diseases - epidemiology
Dog Diseases - parasitology
Dogs
Ecology
Genetic diversity
Genetic Variation
Medical Microbiology
Microbiology
Microscopy
Nigeria
Nigeria - epidemiology
Parasitology
Phylogeny
Piroplasm
RNA, Protozoan - genetics
RNA, Ribosomal, 18S - genetics
spp
Teaching hospitals
Veterinary medicine
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Summary:Adequate knowledge of the genetic diversity among Babesia species infecting dogs is necessary for a better understanding of the epidemiology and control of canine babesiosis. Hence, this study determined the genetic diversity among the Babesia rossi detected in dogs presented for routine examination in Veterinary Hospitals in Abeokuta, Nigeria. Blood were randomly collected from 209 dogs. Field-stained thin smears were made and DNA extracted from the blood. Partial region of the 18S small subunit ribosomal RNA (rRNA) gene was amplified, sequenced and analysed. Babesia species was detected in 16 (7.7%) of the dogs by microscopy. Electrophoresed PCR products from 39 (18.66%) dogs revealed band size of 450 bp and 2 (0.95%) dogs had band size of 430 bp. The sequences obtained from 450 bp amplicon displayed homology of 99.74% (387/388) with partial sequences of 18S rRNA gene of Babesia rossi in the GeneBank. Of the two sequences that had 430 bp amplicon, one was identified as T. annulata and second as T. ovis . A significantly (p
ISSN:1230-2821
1896-1851
DOI:10.1515/ap-2017-0023