Polyclonal emergence of vanA vancomycin-resistant Enterococcus faecium in Australia

To investigate the genetic context associated with the emergence of vanA VRE in Australia. The whole genomes of 18 randomly selected vanA -positive Enterococcus faecium patient isolates, collected between 2011 and 2013 from hospitals in four Australian capitals, were sequenced and analysed. In silic...

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Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 2017-04, Vol.72 (4), p.998-1001
Main Authors: van Hal, Sebastiaan J, Espedido, Björn A, Coombs, Geoffrey W, Howden, Benjamin P, Korman, Tony M, Nimmo, Graeme R, Gosbell, Iain B, Jensen, Slade O
Format: Article
Language:English
Subjects:
Australia - epidemiology
Bacterial Proteins - genetics
Carbon-Oxygen Ligases - genetics
DNA Transposable Elements
Enterococcus faecium - classification
Enterococcus faecium - drug effects
Enterococcus faecium - genetics
Enterococcus faecium - isolation & purification
Genetic Variation
Genome, Bacterial
Gram-Positive Bacterial Infections - epidemiology
Gram-Positive Bacterial Infections - microbiology
Hospitals
Humans
Molecular Typing
Plasmids - analysis
Sequence Analysis, DNA
Vancomycin-Resistant Enterococci - classification
Vancomycin-Resistant Enterococci - genetics
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Summary:To investigate the genetic context associated with the emergence of vanA VRE in Australia. The whole genomes of 18 randomly selected vanA -positive Enterococcus faecium patient isolates, collected between 2011 and 2013 from hospitals in four Australian capitals, were sequenced and analysed. In silico typing and transposon/plasmid assembly revealed that the sequenced isolates represented (in most cases) different hospital-adapted STs and were associated with a variety of different Tn 1546 variants and plasmid backbone structures. The recent emergence of vanA VRE in Australia was polyclonal and not associated with the dissemination of a single 'dominant' ST or vanA -encoding plasmid. Interestingly, the factors contributing to this epidemiological change are not known and future studies may need to consider investigation of potential community sources.
ISSN:0305-7453
1460-2091
DOI:10.1093/jac/dkw539