Molecular diagnosis of Entamoeba spp. versus microscopy in the Great Cairo

Amoebiasis is a human disease produced by Entamoeba histolytica which causes widespread mortality and morbidity worldwide through diarrheal disease and abscess establishment in parenchymal tissues such as liver, lung, and brain. The true prevalence of infection is unknown for most areas of the world...

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Bibliographic Details
Published in:Acta parasitologica 2017-03, Vol.62 (1), p.188-191
Main Authors: Roshdy, Mohamed H., Abd El-Kader, Nour M., Ali-Tammam, Marwa, Fuentes, Isabel, Mohamed, Magdy M., El-Sheikh, Nabila A., Rubio, Jose Miguel
Format: Article
Language:English
Subjects:
Abscesses
Amoebiasis
Animal Systematics/Taxonomy/Biogeography
Biomedical and Life Sciences
Biomedicine
Cairo
Disease
DNA, Protozoan - genetics
Ecology
Egypt
Egypt - epidemiology
Entamoeba - cytology
Entamoeba - genetics
Entamoebiasis - diagnosis
Entamoebiasis - epidemiology
Entamoebiasis - parasitology
Humans
Laboratories
Medical Microbiology
Microbiology
Microscopy
molecular diagnosis
Mortality
Parasitology
Polymerase chain reaction
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Summary:Amoebiasis is a human disease produced by Entamoeba histolytica which causes widespread mortality and morbidity worldwide through diarrheal disease and abscess establishment in parenchymal tissues such as liver, lung, and brain. The true prevalence of infection is unknown for most areas of the world due to the difficulty to characterise Entamoeba histolytica versus other non-pathogenic amoebas with identical morphology, as E ntamoeba dispar , and E ntamoeba moshkovskii . To overcome microscopy misidentification issues, we tested a nested multiplex polymerase chain reaction (PCR) and a real-time PCR on 194 stool samples collected from incoming dysentery patients in Cairo hospitals diagnosed with E. histolytica by microscopy. Nested PCR showed only 20 (10.3%) samples positive to E. histolytica and 17 (8.7%) to E. dispar . The real-time PCR detected only 19 and 11 samples positive to E. histolytica and E. dispar respectively, showing less sensitivity than the nested PCR. The data show that prevalence of E. histolytica in Cairo is lower when specific diagnosis methods are used instead of traditional microscopy, allowing to differentiate between morphologically identical human amoebas species.
ISSN:1230-2821
1896-1851
DOI:10.1515/ap-2017-0022