S-layer protein transport across the cell wall of Corynebacterium glutamicum: in vivo kinetics and energy requirements

Abstract Corynebacteria are Gram-positive bacteria with a very peculiar cell envelope structure as it is constituted of an inner membrane and an outer membrane-like structure. Protein secretion in Corynebacterium glutamicum was studied in vivo, using the S-layer protein PS2 as a model. We show that...

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Bibliographic Details
Published in:FEMS microbiology letters 2002-11, Vol.217 (1), p.71-79
Main Authors: Houssin, C., Nguyen, D.T., Leblon, G., Bayan, N.
Format: Article
Language:English
Subjects:
Bacteria
Bacterial Proteins - classification
Bacterial Proteins - metabolism
Bacteriology
Biological and medical sciences
Carbonyl Cyanide m-Chlorophenyl Hydrazone - agonists
Carbonyl Cyanide m-Chlorophenyl Hydrazone - metabolism
Cell Wall - metabolism
Cell walls
Corynebacterium - metabolism
Corynebacterium glutamicum
Corynomycolic acid
Energy requirements
Energy sources
Fundamental and applied biological sciences. Psychology
Gram-positive bacteria
Kinetics
Membrane Glycoproteins
Membrane proteins
Membranes
Microbiology
Models, Genetic
Permeability, membrane transport, intracellular transport
Presenilin 2
Protein secretion
Protein structure
Protein Transport
Proteins
Proton motive force
Protonmotive force
Secretion
Sodium Azide - agonists
Sodium Azide - metabolism
Time Factors
Translocation
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Summary:Abstract Corynebacteria are Gram-positive bacteria with a very peculiar cell envelope structure as it is constituted of an inner membrane and an outer membrane-like structure. Protein secretion in Corynebacterium glutamicum was studied in vivo, using the S-layer protein PS2 as a model. We show that different variants of PS2 protein are exported through the whole cell envelope with a half-life ranging between 2 and 4 min, by a two-step mechanism. The first step, which is over after about 1.5 min, is ATP- and proton motive force-dependent and may correspond to translocation across the inner membrane via the ‘Sec’ machinery. The second step, across the cell wall and the outer mycolate layer, is rapid but independent of energy sources. This very efficient secretion process across the mycolate layer raises the question of the existence in this layer of a specific machinery.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2002.tb11458.x