Molecular cytogenetic characterization of two established ESFT cell lines

Ewing’s sarcoma/primitive neuroectodermal tumor/Askin’s tumor (Ewing`s sarcoma family of tumors: ESFT) is the most common type of malignant tumor of bone and soft tissue in children and young adults, and morphologically is a member of a group of small round cell tumors. We report, here, on the estab...

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Bibliographic Details
Published in:Human cell : official journal of Human Cell Research Society 2017-01, Vol.30 (1), p.41-48
Main Authors: Ishiguro, Masako, Yuki, Mutsumi, Fukushige, Tomoko, Mizoguchi, Mikio, Kaneko, Yasuhiko, Morishige, Takeshita, Iwasaki, Hiroshi
Format: Article
Language:English
Subjects:
Adult
Animals
Biomedical and Life Sciences
Cell Biology
Cell Line
Cell Line, Tumor
Cytogenetic Analysis
Female
Gynecology
Humans
Immune response
Immune system
Karyotype
Life Sciences
Lymphocytes T
Male
Mice, Inbred BALB C
Neoplasm Transplantation
Oncology
Reproductive Medicine
Reverse Transcriptase Polymerase Chain Reaction
Sarcoma, Ewing - genetics
Sarcoma, Ewing - pathology
Stem Cells
Surgery
Xenografts
Young Adult
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Summary:Ewing’s sarcoma/primitive neuroectodermal tumor/Askin’s tumor (Ewing`s sarcoma family of tumors: ESFT) is the most common type of malignant tumor of bone and soft tissue in children and young adults, and morphologically is a member of a group of small round cell tumors. We report, here, on the establishment of two human ESFT cell lines, FU-PNET-3 and FU-PNET-4, from the iliac and the chest wall, respectively, the cells of both cell lines were tumorigenic in immunodeficient mice. Histologically, both original and xenograft tumors and cultured cells were composed of small round cells with positive immunoreactivity for CD99 and Nkx2.2. Molecular biological examination demonstrated chimeric transcripts of EWSR1 exon 7 to FLI1 exon 6 in FU-PNET-3 cells, and EWSR1 exon 10 to FLI1 exon 6 in FU-PNET-4 cells. Cytogenetic analysis revealed chromosome translocation t(11;22)(q24;q12) and some secondary changes in both cultured cells. These histological, molecular biological, and cytogenetical findings indicate ESFT in both cell lines. ESFT is well studied, but its recurrent fusion genes are heterogeneous and its biological behaviors are unclear. The FU-PNET-3 and FU-PNET-4 cell lines have been well examined and may become useful tools for studying the genetic and biological behavioral properties of ESFT.
ISSN:1749-0774
0914-7470
1749-0774
DOI:10.1007/s13577-016-0145-7