First description of Cryptosporidium hominis GP60 genotype IkA20G1 and Cryptosporidium parvum GP60 genotypes IIaA18G3R1 and IIaA15G2R1 in foals in Brazil

The present study focuses on Cryptosporidium infections of foals in Brazil. A total of 92 animals of different breeds from 11 farms in the vicinity of Araçatuba in the state of São Paulo, were examined. According to PCR targeting the 18S rRNA gene, Cryptosporidium sp. DNA was detected in 21.7% (20/9...

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Bibliographic Details
Published in:Veterinary parasitology 2017-01, Vol.233, p.48-51
Main Authors: Inácio, Sandra Valéria, Widmer, Giovanni, de Brito, Roberta Lomonte Lemos, Zucatto, Anaiza Simão, de Aquino, Monally Conceição Costa, Oliveira, Bruno César Miranda, Nakamura, Alex Akira, Neto, Luiz da Silveira, Carvalho, João Gabriel Balizardo, Gomes, Jancarlo Ferreira, Meireles, Marcelo Vasconcelos, Bresciani, Katia Denise Saraiva
Format: Article
Language:English
Subjects:
Animals
Brazil
Cryptosporidiosis - parasitology
Cryptosporidium - genetics
Cryptosporidium parvum - genetics
DNA, Protozoan - genetics
Equine
Genotype
Horses
HSP70 Heat-Shock Proteins - genetics
Molecular biology
Parasite
RNA, Ribosomal, 18S - genetics
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Summary:The present study focuses on Cryptosporidium infections of foals in Brazil. A total of 92 animals of different breeds from 11 farms in the vicinity of Araçatuba in the state of São Paulo, were examined. According to PCR targeting the 18S rRNA gene, Cryptosporidium sp. DNA was detected in 21.7% (20/92) of foals. Good quality 18S rRNA, actin, HSP70 and gp60 genes nPCR amplicons were obtained from five fecal samples. PCR amplification and sequencing of a fragment of the GP60 sporozoite surface glycoprotein gene revealed C. parvum genotypes IIaA18G3R1, IIaA15G2R1. Interestingly, we also detected in two foals a GP60 genotype related to the human parasite C. hominis.
ISSN:0304-4017
1873-2550
DOI:10.1016/j.vetpar.2016.11.021