Influence of Biodentine registered - A Dentine Substitute - On Collagen Type I Synthesis in Pulp Fibroblasts In Vitro

• Published in: PloS one 2016-12, Vol.11 (12)
• Main Authors: Nikfarjam, Frangis, Beyer, Kim, Konig, Anke, Hofmann, Matthias, Butting, Manuel, Valesky, Eva, Kippenberger, Stefan, Kaufmann, Roland, Heidemann, Detlef, Bernd, August
• Format: Article
• Language: English
• ISSN: 1932-6203
• DOI: 10.1371/journal.pone.0167633

Preserving a patient's own teeth-even in a difficult situation-is nowadays preferable to surgical intervention and therefore promotes development of suitable dental repair materials. Biodentine registered , a mineral trioxide aggregate substitute, has been used to replace dentine in a bioactive and biocompatible manner in both the dental crown and the root. The aim of our study was to evaluate the influence of Biodentine registered on pulp fibroblasts in vitro. For this study, one to five Biodentine registered discs with a diameter of 5.1mm were incubated in DMEM. To obtain Biodentine registered suspensions the media were collected and replaced with fresh medium every 24h for 4 days. Primary pulp cells were isolated from freshly extracted wisdom teeth of 20-23 year old patients and incubated with the Biodentine registered suspensions. Proliferation, cell morphology, cell integrity and cell viability were monitored. To evaluate the effect of Biodentine registered on collagen type I synthesis, the secretion of the N-terminal domain of pro-collagen type I (P1NP) and the release of transforming growth factor-[Beta]1 (TGF-[Beta]1) were quantified. None of the Biodentine registered suspensions tested influenced cell morphology, proliferation or cell integrity. The cell viability varied slightly depending on the suspension used. However, the concentrations of P1NP of all pulp fibroblast cultures treated for 24h with the moderate to high Biodentine registered concentration containing suspensions of day 1 were reduced to 5% of the control. Furthermore, a significant TGF-[Beta]1 reduction was observed after treatment with these suspensions. It could be shown that Biodentine registered is biocompatible. However, dissolved particles of the moderate to high concentrated Biodentine registered suspensions 24h after mixing induce a significant reduction of TGF-[Beta]1 release and reduce the secretion of collagen type I of primary pulp fibroblasts.