Host plant growth promotion and cadmium detoxification in Solanum nigrum, mediated by endophytic fungi

• Published in: Ecotoxicology and environmental safety 2017-02, Vol.136, p.180-188
• Main Authors: Khan, Abdur Rahim, Ullah, Ihsan, Waqas, Muhammad, Park, Gun-Seok, Khan, Abdul Latif, Hong, Sung-Jun, Ullah, Rehman, Jung, Byung Kwon, Park, Chang Eon, Ur-Rehman, Shafiq, Lee, In-Jung, Shin, Jae-Ho
• Format: Article
• Language: English
• Subjects: Biodegradation, Environmental; Cadmium - metabolism; Cd-hyperaccumulator; Endophytes - physiology; Endophytic fungi; Fungi - physiology; Inactivation, Metabolic; Phytoremediation; Soil Pollutants - metabolism; Solanum nigrum; Solanum nigrum - drug effects; Solanum nigrum - growth & development; Solanum nigrum - metabolism; Solanum nigrum - microbiology
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2016.03.014

Current investigation conducted to evaluate the associated fungal endophyte interactions of a Cd hyper-accumulator Solanum nigrum Korean ecotype under varying concentrations of Cd. Two indole-3-acetic acid (IAA) producing fungal strains, RSF-4L and RSF-6L, isolated from the leaves of S. nigrum, were initially screened for Cd tolerance and accumulation potential. In terms of dry biomass production, the strain RSF-6L showed higher tolerance and accumulation capacity for Cd toxicity in comparison to RSF-4L. Therefore, RSF-6L was applied in vivo to S. nigrum and grown for six weeks under Cd concentrations of 0, 10, and 30mgKg−1 of dry sand. The effect of fungal inoculation assessed by plant physiological responses, endogenous biochemical regulations, and Cd profile in different tissues. Significant increase were observed in plant growth attributes such as shoot length, root length, dry biomass, leaf area, and chlorophyll contents in inoculated RSF-6L plants in comparison to non-inoculated plants with or without Cd contamination. RSF-6L inoculation decreased uptake of Cd in roots and above ground parts, as evidenced by a low bio-concentration factor (BCF) and improved tolerance index (TI). However, Cd concentration in the leaves remained the same for inoculated and non-inoculated plants under Cd spiking. Fungal inoculation protected the host plants, as evidenced by low peroxidase (POD) and polyphenol peroxidase (PPO) activities and high catalase (CAT) activity. Application of appropriate fungal inoculation that can improve tolerance mechanisms of hyper-accumulators and reduce Cd uptake can be recommended for phyto-stabilisation/immobilisation of heavy metals in crop fields. •Integrated plant-microbe phytoremediation is gaining attraction worldwide.•The host plant was inoculated with fungal endophyte RSF-6L under Cd contamination.•Cd contamination significantly retards plant growth attributes.•Inoculation with the fungal strain RSF-6L increases the growth parameters.