HLA-G profile of infertile couples who underwent assisted reproduction treatment

Abstract HLA-G codes for a non-classical Class I (Ib) protein which is mainly expressed in trophoblast cells. Many pieces of evidence pointed out its essential role conferring immunological tolerance to the fetus. Some HLA-G alleles have been linked to enhanced or reduced HLA-G protein levels expres...

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Bibliographic Details
Published in:Human immunology 2016-12, Vol.77 (12), p.1179-1186
Main Authors: Hernandes Costa, Cynthia, Fernanda Gelmini, Georgia, Silva Nardi, Fabiola, Maria Munhoz Sperandio Roxo, Valéria, Schuffner, Alessandro, da Graça Bicalho, Maria
Format: Article
Language:English
Subjects:
Adult
Allergy and Immunology
Brazil
Female
Gene Frequency
Genetic Association Studies
Genotype
HLA-G Antigens - genetics
HLA-G coding region
HLA-G regulatory region
Human reproduction
Humans
Infertility - genetics
Infertility - therapy
Male
Middle Aged
Pregnancy
Protective haplotype
Reproductive Techniques, Assisted
Susceptibility haplotype
Young Adult
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Summary:Abstract HLA-G codes for a non-classical Class I (Ib) protein which is mainly expressed in trophoblast cells. Many pieces of evidence pointed out its essential role conferring immunological tolerance to the fetus. Some HLA-G alleles have been linked to enhanced or reduced HLA-G protein levels expression, which have been associated with reproductive failure. In this study 33 couples undergoing ART (assisted reproduction treatment; n = 66) and 120 couples who conceived naturally (controls; n = 240) were enrolled in the study. Genotyping was performed by SBT and tagged an 1837 bp at 5́URR as well as exons 2, 3 and 4 of HLA-G . Alleles, genotypes and haplotypes were compared between infertile and control groups using Fisher Exact Test. The haplotype HLA-G∗010101b/HLA-G∗01:01:01 showed statistically significant higher frequency in control groups. The immunogenetics of infertility is complex and might be dependent on different genes involved in the establishment of a successful pregnancy. A better understanding of HLA-G alleles and haplotypes structure and how the genetic diversity at their regulatory sites could impact on their level of expression and build up the susceptibility or protection conditions may shed light on the comprehension of immunogenetics mechanisms acting at the fetus-maternal interface.
ISSN:0198-8859
1879-1166
DOI:10.1016/j.humimm.2016.09.002