Size-Dependent Cellular Uptake of DNA Functionalized Gold Nanoparticles

The extensive use of gold nanoparticles (AuNPs) in nanomedicine, especially for intracellular imaging, photothermal therapy, and drug delivery, has necessitated the study of how functionalized AuNPs engage with living biological interfaces like the mammalian cell. Nanoparticle size, shape, surface c...

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Bibliographic Details
Published in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2016-10, Vol.12 (40), p.5592-5600
Main Authors: Wong, Alexis C., Wright, David W.
Format: Article
Language:English
Subjects:
Cell Line, Tumor
cellular uptake
Cytometry
Deoxyribonucleic acid
Design analysis
DNA
DNA - chemistry
Drug delivery systems
Endocytosis
Flow cytometry
Fluorescence
Gold
Gold - chemistry
gold nanoparticles
Humans
ICP-MS
Imaging
Inductively coupled plasma mass spectrometry
Mass spectrometry
Metal Nanoparticles - chemistry
Nanoparticles
Nanostructure
Nanotechnology
Particle Size
Spectrophotometry, Atomic
Surface charge
Uptakes
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Summary:The extensive use of gold nanoparticles (AuNPs) in nanomedicine, especially for intracellular imaging, photothermal therapy, and drug delivery, has necessitated the study of how functionalized AuNPs engage with living biological interfaces like the mammalian cell. Nanoparticle size, shape, surface charge, and surface functionality can affect the accumulation of functionalized AuNPs in cells. Confocal microscopy, flow cytometry, and inductively coupled plasma mass spectrometry demonstrate that CaSki cells, a human cervical cancer cell line, internalize AuNPs functionalized with hairpin, single stranded, and double stranded DNA differently. Surface charge and DNA conformation are shown to have no effect on the cell‐nanoparticle interaction. CaSki cells accumulate small DNA‐AuNPs in greater quantities than large DNA‐AuNPs, demonstrating that size is the major contributor to cellular uptake properties. These data suggest that DNA‐AuNPs can be easily tailored through modulation of size to design functional AuNPs with optimal cellular uptake properties and enhanced performance in nanomedicine applications. Systematic variation of DNA conformation, DNA length, and nanoparticle core diameter is performed to evaluate the influence of size, surface charge, and DNA conformation on the internalization of DNA functionalized gold nanoparticles by cells. Cellular uptake is primarily dependent on size, which informs the design of gold nanoparticle probes that possess enhanced cell entry properties for imaging and therapeutic applications.
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.201601697