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Multigene Assessment of Biodiversity of Diatom(Bacillariophyceae) Assemblages from the Littoral Zone of the Bohai and Yellow Seas in Yantai Region of Northeast China with some Remarks on Ubiquitous Taxa
Diatoms are important contributors to the benthic micro-eukaryote flora. This manuscript lays the foundation for future meta-genomic and environmental sequencing projects off coastal China by curating diatom DNA sequences from the Yantai region of the Bohai and Yellow Seas (Northeast China). These s...
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Published in: | Journal of coastal research 2016-01, Vol.SI (74), p.166-166 |
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creator | Witkowski, Andrzej Li, Chunlian Zglobicka, Izabela Yu, Shu-xian Ashworth, Matt Dabek, Przemyslaw Qin, Song Tang, Cheng Krzywda, Marta Ruppel, Manfred Theriot, Edward C Jansen, Robert K Car, Ana Plocinski, Tomasz Wang, Yin-chu Sabir, Jamal S M Daniszewska-Kowalczyk, Genowefa Kierzek, Agnieszka Hajrah, Nahid H |
description | Diatoms are important contributors to the benthic micro-eukaryote flora. This manuscript lays the foundation for future meta-genomic and environmental sequencing projects off coastal China by curating diatom DNA sequences from the Yantai region of the Bohai and Yellow Seas (Northeast China). These studies are based on cultures established from samples collected in different seasons from marine littoral and supra-littoral zones in 2013 and 2014. Thirty-six diatom strains were cultured successfully and identification of these clones was determined by light and scanning electron microscopy(LM and SEM) and DNA sequencing of the nuclear-encoded small subunit ribosomal RNA (SSU)and chloroplast-encoded rbcL and psbC genes. The strains primarily represent raphid pennate genera, such as Amphora, Amphora (Oxyamphora), Caloneis, Diploneis, Halamphora, Navicula, Nitzschia, Parlibellus, Pleurosigma, Surirella and Tryblionella. The authors also compared the molecular and morphological identities of several seemingly ubiquitous marine littoral taxa in the genera Amphora and Nitzschia from the Indian Ocean and Atlantic Ocean, the Red Sea and Adriatic Sea to their Yellow Sea counterparts. |
doi_str_mv | 10.2112/SI74-016 |
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This manuscript lays the foundation for future meta-genomic and environmental sequencing projects off coastal China by curating diatom DNA sequences from the Yantai region of the Bohai and Yellow Seas (Northeast China). These studies are based on cultures established from samples collected in different seasons from marine littoral and supra-littoral zones in 2013 and 2014. Thirty-six diatom strains were cultured successfully and identification of these clones was determined by light and scanning electron microscopy(LM and SEM) and DNA sequencing of the nuclear-encoded small subunit ribosomal RNA (SSU)and chloroplast-encoded rbcL and psbC genes. The strains primarily represent raphid pennate genera, such as Amphora, Amphora (Oxyamphora), Caloneis, Diploneis, Halamphora, Navicula, Nitzschia, Parlibellus, Pleurosigma, Surirella and Tryblionella. The authors also compared the molecular and morphological identities of several seemingly ubiquitous marine littoral taxa in the genera Amphora and Nitzschia from the Indian Ocean and Atlantic Ocean, the Red Sea and Adriatic Sea to their Yellow Sea counterparts.</description><identifier>ISSN: 0749-0208</identifier><identifier>EISSN: 1551-5036</identifier><identifier>DOI: 10.2112/SI74-016</identifier><language>eng</language><publisher>Fort Lauderdale: Allen Press Inc</publisher><subject>Adriatic Sea ; Amphora ; Assessments ; Bacillariophyceae ; Biodiversity ; Caloneis ; Cloning ; Coasts ; Deoxyribonucleic acid ; Diploneis ; DNA ; Flora ; Flow velocity ; Gene sequencing ; Islands ; Littoral environments ; Littoral zone ; Metadata ; Microorganisms ; Microscopy ; Morphology ; Navicula ; Nitzschia ; Plankton ; Pleurosigma ; Ribonucleic acids ; Salinity ; Scanning electron microscopy ; Science ; Seas ; Studies ; Surirella ; Taxa ; Tryblionella</subject><ispartof>Journal of coastal research, 2016-01, Vol.SI (74), p.166-166</ispartof><rights>Copyright Allen Press Publishing Services 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Witkowski, Andrzej</creatorcontrib><creatorcontrib>Li, Chunlian</creatorcontrib><creatorcontrib>Zglobicka, Izabela</creatorcontrib><creatorcontrib>Yu, Shu-xian</creatorcontrib><creatorcontrib>Ashworth, Matt</creatorcontrib><creatorcontrib>Dabek, Przemyslaw</creatorcontrib><creatorcontrib>Qin, Song</creatorcontrib><creatorcontrib>Tang, Cheng</creatorcontrib><creatorcontrib>Krzywda, Marta</creatorcontrib><creatorcontrib>Ruppel, Manfred</creatorcontrib><creatorcontrib>Theriot, Edward C</creatorcontrib><creatorcontrib>Jansen, Robert K</creatorcontrib><creatorcontrib>Car, Ana</creatorcontrib><creatorcontrib>Plocinski, Tomasz</creatorcontrib><creatorcontrib>Wang, Yin-chu</creatorcontrib><creatorcontrib>Sabir, Jamal S M</creatorcontrib><creatorcontrib>Daniszewska-Kowalczyk, Genowefa</creatorcontrib><creatorcontrib>Kierzek, Agnieszka</creatorcontrib><creatorcontrib>Hajrah, Nahid H</creatorcontrib><title>Multigene Assessment of Biodiversity of Diatom(Bacillariophyceae) Assemblages from the Littoral Zone of the Bohai and Yellow Seas in Yantai Region of Northeast China with some Remarks on Ubiquitous Taxa</title><title>Journal of coastal research</title><description>Diatoms are important contributors to the benthic micro-eukaryote flora. This manuscript lays the foundation for future meta-genomic and environmental sequencing projects off coastal China by curating diatom DNA sequences from the Yantai region of the Bohai and Yellow Seas (Northeast China). These studies are based on cultures established from samples collected in different seasons from marine littoral and supra-littoral zones in 2013 and 2014. Thirty-six diatom strains were cultured successfully and identification of these clones was determined by light and scanning electron microscopy(LM and SEM) and DNA sequencing of the nuclear-encoded small subunit ribosomal RNA (SSU)and chloroplast-encoded rbcL and psbC genes. The strains primarily represent raphid pennate genera, such as Amphora, Amphora (Oxyamphora), Caloneis, Diploneis, Halamphora, Navicula, Nitzschia, Parlibellus, Pleurosigma, Surirella and Tryblionella. The authors also compared the molecular and morphological identities of several seemingly ubiquitous marine littoral taxa in the genera Amphora and Nitzschia from the Indian Ocean and Atlantic Ocean, the Red Sea and Adriatic Sea to their Yellow Sea counterparts.</description><subject>Adriatic Sea</subject><subject>Amphora</subject><subject>Assessments</subject><subject>Bacillariophyceae</subject><subject>Biodiversity</subject><subject>Caloneis</subject><subject>Cloning</subject><subject>Coasts</subject><subject>Deoxyribonucleic acid</subject><subject>Diploneis</subject><subject>DNA</subject><subject>Flora</subject><subject>Flow velocity</subject><subject>Gene sequencing</subject><subject>Islands</subject><subject>Littoral environments</subject><subject>Littoral 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Edward C</au><au>Jansen, Robert K</au><au>Car, Ana</au><au>Plocinski, Tomasz</au><au>Wang, Yin-chu</au><au>Sabir, Jamal S M</au><au>Daniszewska-Kowalczyk, Genowefa</au><au>Kierzek, Agnieszka</au><au>Hajrah, Nahid H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multigene Assessment of Biodiversity of Diatom(Bacillariophyceae) Assemblages from the Littoral Zone of the Bohai and Yellow Seas in Yantai Region of Northeast China with some Remarks on Ubiquitous Taxa</atitle><jtitle>Journal of coastal research</jtitle><date>2016-01-01</date><risdate>2016</risdate><volume>SI</volume><issue>74</issue><spage>166</spage><epage>166</epage><pages>166-166</pages><issn>0749-0208</issn><eissn>1551-5036</eissn><abstract>Diatoms are important contributors to the benthic micro-eukaryote flora. This manuscript lays the foundation for future meta-genomic and environmental sequencing projects off coastal China by curating diatom DNA sequences from the Yantai region of the Bohai and Yellow Seas (Northeast China). These studies are based on cultures established from samples collected in different seasons from marine littoral and supra-littoral zones in 2013 and 2014. Thirty-six diatom strains were cultured successfully and identification of these clones was determined by light and scanning electron microscopy(LM and SEM) and DNA sequencing of the nuclear-encoded small subunit ribosomal RNA (SSU)and chloroplast-encoded rbcL and psbC genes. The strains primarily represent raphid pennate genera, such as Amphora, Amphora (Oxyamphora), Caloneis, Diploneis, Halamphora, Navicula, Nitzschia, Parlibellus, Pleurosigma, Surirella and Tryblionella. The authors also compared the molecular and morphological identities of several seemingly ubiquitous marine littoral taxa in the genera Amphora and Nitzschia from the Indian Ocean and Atlantic Ocean, the Red Sea and Adriatic Sea to their Yellow Sea counterparts.</abstract><cop>Fort Lauderdale</cop><pub>Allen Press Inc</pub><doi>10.2112/SI74-016</doi><tpages>1</tpages></addata></record> |
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subjects | Adriatic Sea Amphora Assessments Bacillariophyceae Biodiversity Caloneis Cloning Coasts Deoxyribonucleic acid Diploneis DNA Flora Flow velocity Gene sequencing Islands Littoral environments Littoral zone Metadata Microorganisms Microscopy Morphology Navicula Nitzschia Plankton Pleurosigma Ribonucleic acids Salinity Scanning electron microscopy Science Seas Studies Surirella Taxa Tryblionella |
title | Multigene Assessment of Biodiversity of Diatom(Bacillariophyceae) Assemblages from the Littoral Zone of the Bohai and Yellow Seas in Yantai Region of Northeast China with some Remarks on Ubiquitous Taxa |
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