Fluorescent zinc(ii) complexes for gene delivery and simultaneous monitoring of protein expression

Two new zinc(ii) complexes, [Zn(l-His)(NIP)] (1) and [Zn(acac) (NIP)](2) (where NIP is 2-(naphthalen-1-yl)-1H-imidazo[4,5-f][1,10]phenanthroline, acac = acetyl acetone), have been synthesized and characterized by elemental analysis, UV-vis, fluorescence, IR, H NMR and electron spray ionization mass...

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Bibliographic Details
Published in:Dalton transactions : an international journal of inorganic chemistry 2016-01, Vol.45 (42), p.16984-16996
Main Authors: Rane, Lalita B, Kate, Anup N, Ramteke, Shefali N, Shravage, Bhupendra V, Kulkarni, Prasad P, Kumbhar, Anupa A
Format: Article
Language:English
Subjects:
Condensation
Coordination Complexes - chemistry
Coordination compounds
Deoxyribonucleic acid
DNA, Circular - administration & dosage
DNA, Circular - genetics
EDTA
Fluorescence
Fluorescent Dyes - chemistry
Gene Expression
Gene Transfer Techniques
Humans
Imidazoles - chemistry
Ionization
Luminescent Proteins - genetics
MCF-7 Cells
Microscopy, Confocal
Nanostructure
Naphthalenes - chemistry
Optical Imaging
Phenanthrolines - chemistry
Plasmids - administration & dosage
Plasmids - genetics
Proteins
Red Fluorescent Protein
Transfection - methods
Zinc - chemistry
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Summary:Two new zinc(ii) complexes, [Zn(l-His)(NIP)] (1) and [Zn(acac) (NIP)](2) (where NIP is 2-(naphthalen-1-yl)-1H-imidazo[4,5-f][1,10]phenanthroline, acac = acetyl acetone), have been synthesized and characterized by elemental analysis, UV-vis, fluorescence, IR, H NMR and electron spray ionization mass spectroscopies. Gel retardation assay, atomic force microscopy and dynamic light scattering studies show that 1 and 2 can induce the condensation of circular plasmid pBR322 DNA into nanometer size particles under ambient conditions. Treatment of 2 with 5 mM EDTA restored 30% of the supercoiled form of DNA, revealing partial reversibility of DNA condensation. The in vitro transfection experiment demonstrates that the complexes can be used to deliver pCMV-tdTomato-N1 plasmid which expresses red fluorescent protein. The confocal studies show that the fluorescent nature of complexes is advantageous for visualizing the intracellular delivery of metal complexes as well as transfection efficiency using two distinct emission windows.
ISSN:1477-9226
1477-9234
DOI:10.1039/c6dt02871c