Evaluation of vascular endothelial growth factor gene and protein expression in canine metastatic mammary carcinomas

Vascular endothelial growth factor (VEGF) is a potent angiogenic factor that could be associated with the induction of endothelial cell proliferation and metastasis. In this study, we evaluated VEGF gene and protein expression in canine mammary tumors (CMT), including metastatic carcinomas, to deter...

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Bibliographic Details
Published in:Microscopy research and technique 2016-11, Vol.79 (11), p.1097-1104
Main Authors: Raposo-Ferreira, Talita M.M., Salvador, Rosana C.L., Terra, Erika M., Ferreira, Juarez H., Vechetti-Junior, Ivan José, Tinucci-Costa, Mirela, Rogatto, Silvia R., Laufer-Amorim, Renée
Format: Article
Language:English
Subjects:
Animals
Brazil
Cancer
Correlation
dog
Dogs
Female
Gene expression
Genes
Growth factors
Immunohistochemistry
mammary gland
Mammary glands
Mammary Glands, Animal - chemistry
Mammary Glands, Animal - metabolism
Mammary Glands, Animal - pathology
Mammary Neoplasms, Animal - chemistry
Mammary Neoplasms, Animal - genetics
Mammary Neoplasms, Animal - metabolism
Mammary Neoplasms, Animal - pathology
Metastasis
Polymerase Chain Reaction
Protein expression
Proteins
tumor
Tumors
Vascular endothelial growth factor
Vascular Endothelial Growth Factor A - analysis
Vascular Endothelial Growth Factor A - genetics
Vascular Endothelial Growth Factor A - metabolism
VEGF
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Summary:Vascular endothelial growth factor (VEGF) is a potent angiogenic factor that could be associated with the induction of endothelial cell proliferation and metastasis. In this study, we evaluated VEGF gene and protein expression in canine mammary tumors (CMT), including metastatic carcinomas, to determine if there is an influence of this marker in the malignant processes and aggressiveness of CMT. We also compared VEGF protein levels with clinicopathological features. The VEGF gene and protein expression levels were evaluated by reverse transcription‐quantitative polymerase chain reaction and immunohistochemistry, respectively, in normal mammary gland samples, benign mammary tumors, nonmetastatic mammary carcinomas, and metastatic mammary carcinomas. High VEGF gene and protein levels were associated with malignant tumors compared with normal mammary glands (p = 0.0089 and p 
ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.22763