A Simple PCR-RFLP Method for Genotyping of IFNL4 rs368234815 Polymorphism in Patients With Chronic Hepatitis C

• Published in: Laboratory medicine 2017-02, Vol.48 (1), p.51-56
• Main Authors: Pouryasin, Mohammad, Sharafi, Heidar, Behnava, Bita, Alavian, Seyed Moayed, Keshvari, Maryam, Pouryasin, Ali
• Format: Article
• Language: English
• Subjects: Adult; Cohort Studies; Female; Genotype & phenotype; Genotyping Techniques - methods; Hepatitis; Hepatitis C; Hepatitis C, Chronic - genetics; Humans; Interleukins - genetics; Male; Patients; Polymerase chain reaction; Polymerase Chain Reaction - methods; Polymorphism; Polymorphism, Restriction Fragment Length - genetics; Polymorphism, Single Nucleotide - genetics; Young Adult
• ISSN: 0007-5027; 1943-7730
• DOI: 10.1093/labmed/lmw060

The IFNL4 rs368234815 polymorphism plays a prominent role in spontaneous and treatment-induced clearance of hepatitis C virus (HCV) infection. This study aimed to develop a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for assessment of rs368234815 polymorphism. We genotyped the rs368234815 polymorphism in 87 patients with chronic HCV by PCR sequencing and PCR-RFLP methods, simultaneously. Genotyping of IFNL4 rs368234815 via PCR-RFLP was concordant with PCR sequencing in all 87 individuals (100%). The analytical sensitivity and specificity of the developed PCR-RFLP method for genotyping of rs368234815 polymorphism were each 100%. Among these patients with chronic HCV, the frequency of rs368234815 TT/TT, TT/ΔG, and ΔG/ΔG were 44.8%, 37.9%, and 17.3%, respectively. The PCR-RFLP method that we developed is accurate, rapid, inexpensive, and easy to perform for genotyping of the IFNL4 rs368234815 polymorphism. This method can be used for clinical and research work.