Comparison of Xpert Norovirus and RidaGene Norovirus assays for the detection of noroviruses in clinical fecal specimens

The purpose of this study was to investigate the usability and performance of the Xpert Norovirus and RidaGene Norovirus assays for the detection of noroviruses in fecal specimens. Of the 186 stool specimens, 53 (28.5%) were considered true-positive for norovirus (NoV). Of the true-positive specimen...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 2017-06, Vol.36 (6), p.1019-1022
Main Authors: Aho-Laukkanen, E., Hirvonen, J. J., Saha, K.
Format: Article
Language:English
Subjects:
Assaying
Biomedical and Life Sciences
Biomedicine
Caliciviridae Infections - diagnosis
Caliciviridae Infections - virology
Confidence intervals
Feces - virology
Humans
Internal Medicine
Medical Microbiology
Molecular Diagnostic Techniques - methods
Norovirus - genetics
Norovirus - isolation & purification
Original Article
Predictive Value of Tests
Sensitivity and Specificity
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Summary:The purpose of this study was to investigate the usability and performance of the Xpert Norovirus and RidaGene Norovirus assays for the detection of noroviruses in fecal specimens. Of the 186 stool specimens, 53 (28.5%) were considered true-positive for norovirus (NoV). Of the true-positive specimens, Xpert detected 53 and RidaGene detected 52. The respective sensitivity and specificity were 100% and 94.7% [95% confidence interval (CI), 91.0–98.5%] for the Xpert assay, and 98.1% (95% CI, 94.4–100%) and 97.0% (95% CI, 94.1–99.9%) for the RidaGene assay. Positive and negative predictive values (PPVs and NPVs) were 88.3% and 100% for the Xpert assay, and 92.9% and 99.2% for the RidaGene assay, respectively. Based on this study, it can be concluded that there were no significant differences ( p -value > 0.5) between the results of the Xpert and RidaGene Norovirus assays. We found that both assays are useful for the detection of noroviruses in clinical stool samples.
ISSN:0934-9723
1435-4373
DOI:10.1007/s10096-016-2884-5