Presence of osteoclasts in middle ear cholesteatoma: a study of undecalcified bone sections

Conclusions: Osteoclasts are unlikely to be involved in bone resorption in middle ear cholesteatoma. Objective: The authors searched for osteoclasts in undecalcified bone sections in patients with middle ear cholesteatoma to determine whether and to what extent these cells are involved in this disea...

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Published in:Acta oto-laryngologica 2017-02, Vol.137 (2), p.127-130
Main Authors: Koizumi, Hiroki, Suzuki, Hideaki, Kawaguchi, Rintaro, Hashida, Koichi, Hohchi, Nobusuke, Ohkubo, Jun-Ichi, Tabata, Takahisa, Kitamura, Takuro
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
bone resorption
Cholesteatoma, Middle Ear - pathology
Female
Humans
Male
Middle Aged
Middle ear cholesteatoma
osteoclast
Osteoclasts
scutum bone
Temporal Bone - pathology
undecalcified bone section
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cited_by cdi_FETCH-LOGICAL-c432t-8ab00bba7ef7b8233728c5aa47ca0bb514f864fdb4c4027c3e0182eddcfc26933
cites cdi_FETCH-LOGICAL-c432t-8ab00bba7ef7b8233728c5aa47ca0bb514f864fdb4c4027c3e0182eddcfc26933
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container_issue 2
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container_title Acta oto-laryngologica
container_volume 137
creator Koizumi, Hiroki
Suzuki, Hideaki
Kawaguchi, Rintaro
Hashida, Koichi
Hohchi, Nobusuke
Ohkubo, Jun-Ichi
Tabata, Takahisa
Kitamura, Takuro
description Conclusions: Osteoclasts are unlikely to be involved in bone resorption in middle ear cholesteatoma. Objective: The authors searched for osteoclasts in undecalcified bone sections in patients with middle ear cholesteatoma to determine whether and to what extent these cells are involved in this disease. Methods: Twelve patients, eight men and four women, aged 30-87 years, who underwent tympanomastoidectomy were enrolled. Six patients had primary acquired middle ear cholesteatoma (cholesteatoma group) and the other six patients had other otologic diseases including otosclerosis, non-cholesteatomatous chronic otitis media, adhesive otitis media, perilymphatic fistula and ossicular malformation (control group). The scutum bone was collected during surgery, fixed with ethanol, stained with Villanueva bone stain, and embedded in methyl methacrylate. Five-micrometer-thick sections were prepared and examined under a polarizing microscope. Images were analyzed using a semiautomatic graphics system. Results: No osteoclasts were seen in any of the samples in either group. To avoid the risk of under-estimating the presence of osteoclasts, the number of osteoclasts was considered to be
doi_str_mv 10.1080/00016489.2016.1222549
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Objective: The authors searched for osteoclasts in undecalcified bone sections in patients with middle ear cholesteatoma to determine whether and to what extent these cells are involved in this disease. Methods: Twelve patients, eight men and four women, aged 30-87 years, who underwent tympanomastoidectomy were enrolled. Six patients had primary acquired middle ear cholesteatoma (cholesteatoma group) and the other six patients had other otologic diseases including otosclerosis, non-cholesteatomatous chronic otitis media, adhesive otitis media, perilymphatic fistula and ossicular malformation (control group). The scutum bone was collected during surgery, fixed with ethanol, stained with Villanueva bone stain, and embedded in methyl methacrylate. Five-micrometer-thick sections were prepared and examined under a polarizing microscope. Images were analyzed using a semiautomatic graphics system. Results: No osteoclasts were seen in any of the samples in either group. To avoid the risk of under-estimating the presence of osteoclasts, the number of osteoclasts was considered to be &lt;1 in each sample, and the osteoclast density was calculated. The osteoclast densities in both the cholesteatoma and control groups were significantly lower than the sex- and age-matched standard value of the normal iliac cortical bone (p = .028).</description><identifier>ISSN: 0001-6489</identifier><identifier>EISSN: 1651-2251</identifier><identifier>DOI: 10.1080/00016489.2016.1222549</identifier><identifier>PMID: 27575923</identifier><language>eng</language><publisher>England: Taylor &amp; Francis</publisher><subject>Adult ; Aged ; Aged, 80 and over ; bone resorption ; Cholesteatoma, Middle Ear - pathology ; Female ; Humans ; Male ; Middle Aged ; Middle ear cholesteatoma ; osteoclast ; Osteoclasts ; scutum bone ; Temporal Bone - pathology ; undecalcified bone section</subject><ispartof>Acta oto-laryngologica, 2017-02, Vol.137 (2), p.127-130</ispartof><rights>2016 Acta Oto-Laryngologica AB (Ltd) 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c432t-8ab00bba7ef7b8233728c5aa47ca0bb514f864fdb4c4027c3e0182eddcfc26933</citedby><cites>FETCH-LOGICAL-c432t-8ab00bba7ef7b8233728c5aa47ca0bb514f864fdb4c4027c3e0182eddcfc26933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27575923$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koizumi, Hiroki</creatorcontrib><creatorcontrib>Suzuki, Hideaki</creatorcontrib><creatorcontrib>Kawaguchi, Rintaro</creatorcontrib><creatorcontrib>Hashida, Koichi</creatorcontrib><creatorcontrib>Hohchi, Nobusuke</creatorcontrib><creatorcontrib>Ohkubo, Jun-Ichi</creatorcontrib><creatorcontrib>Tabata, Takahisa</creatorcontrib><creatorcontrib>Kitamura, Takuro</creatorcontrib><title>Presence of osteoclasts in middle ear cholesteatoma: a study of undecalcified bone sections</title><title>Acta oto-laryngologica</title><addtitle>Acta Otolaryngol</addtitle><description>Conclusions: Osteoclasts are unlikely to be involved in bone resorption in middle ear cholesteatoma. 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To avoid the risk of under-estimating the presence of osteoclasts, the number of osteoclasts was considered to be &lt;1 in each sample, and the osteoclast density was calculated. 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subjects Adult
Aged
Aged, 80 and over
bone resorption
Cholesteatoma, Middle Ear - pathology
Female
Humans
Male
Middle Aged
Middle ear cholesteatoma
osteoclast
Osteoclasts
scutum bone
Temporal Bone - pathology
undecalcified bone section
title Presence of osteoclasts in middle ear cholesteatoma: a study of undecalcified bone sections
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