Cellular energetics of frozen wood frogs ( Rana sylvatica) revealed via NMR spectroscopy

(1) 1H and 31P NMR spectroscopy were used to measure high energy organophosphates (ATP and creative phosphate, CP) and to estimate intracellular pH in juvenile Rana sylvatica during freezes at –1.5°C lasting up to 7 d. (2) Frogs largely maintained their levels of ATP and CP throughout a slow freeze;...

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Bibliographic Details
Published in:Journal of thermal biology 2002-06, Vol.27 (3), p.167-173
Main Authors: Layne, Jack R., Kennedy, Scott D.
Format: Article
Language:English
Subjects:
Freeze-tolerance
High energy
Intracellular pH
NMR spectroscopy
Organophosphates
Rana sylvatica
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Summary:(1) 1H and 31P NMR spectroscopy were used to measure high energy organophosphates (ATP and creative phosphate, CP) and to estimate intracellular pH in juvenile Rana sylvatica during freezes at –1.5°C lasting up to 7 d. (2) Frogs largely maintained their levels of ATP and CP throughout a slow freeze; whereas, frogs undergoing a fast freeze experienced +80% reductions in ATP and CP within 7 d. (3) Correspondingly, intracellular pH was stable (7.2–7.6) throughout the slow freeze but pH was reduced to 6.57 during the fast freeze. (4) The deleterious effects of harsh freeze conditions may have an energetic underpinning but it is premature to assign a causal role at this point.
ISSN:0306-4565
1879-0992
DOI:10.1016/S0306-4565(01)00022-5