UV Absorbance Dependent Toxicity of Acridine to the Marine Diatom Phaeodactylum tricornutum

• Published in: Environmental science & technology 2002-03, Vol.36 (5), p.908-913
• Main Authors: Wiegman, Saskia, Termeer, Joost A. G, Verheul, Tommie, Kraak, Michiel H. S, de Voogt, Pim, Laane, Remi W. P. M, Admiraal, Wim
• Format: Article
• Language: English
• Subjects: acridine; Acridines - toxicity; Animal, plant and microbial ecology; Applied ecology; Biological and medical sciences; Diatoms; Dose-Response Relationship, Drug; Ecotoxicology, biological effects of pollution; Effects of pollution and side effects of pesticides on plants and fungi; Fundamental and applied biological sciences. Psychology; Marine biology; Phaeodactylum tricornutum; Photochemistry; Toxicity; Toxicity Tests; Ultraviolet radiation; Ultraviolet Rays; Water Pollutants, Chemical - toxicity
• ISSN: 0013-936X; 1520-5851
• DOI: 10.1021/es010149q

The present study seeks quantitative measures for photoenhanced toxicity under natural light regimes by comparing the effects of an aromatic compound under natural and laboratory light. To this purpose, the influence of light irradiance and spectral composition on the extent of photoenhanced toxicity of acridine, a three-ringed azaarene, to the marine diatom Phaeodactylum tricornutum was analyzed. Under laboratory light containing ultraviolet radiation (UV), the 72-h EC50 growth value for acridine was 1.55 μM. Under natural light, a 72-h EC50 value for acridine below the lowest test concentration (0.44 μM) was observed. Under both laboratory and natural light, the toxicity of acridine was equally enhanced by total UV (UV-A and UV-B) and UV-A radiation, while in the absence of UV no enhancement of toxicity was observed. Hence, the UV-A region of light was dominant in the photoenhanced toxicity of acridine to P. tricornutum, in accordance with its absorption spectrum in the UV-A region. Therefore, the total amount of UV radiation absorbed by aqueous acridine was calculated for each separate treatment. The amount of UV absorbed by acridine effectively described the effect of acridine on the growth of P. tricornutum in a dose−response-dependent manner. It is concluded that photoenhanced toxicity of aromatic compounds expressed as a function of the actually absorbed UV may circumvent some of the variability between studies using different concentrations of the phototoxic compounds and light sources. The UV quantity absorbed by these compounds allows a comparison with the absorption characteristics of natural waters and, thus, is a key parameter to determine the role of photoenhanced toxicity in water.