Chromosome Microdissection-Based Techniques for Genome Analysis

It is now possible to start at a cytogenetically defined position in any eucaryotic genome and proceed toward isolation and identification of candidate genes known to map to that position, taking advantage of the new PCR amplification technology to produce position-specific DNA. The starting materia...

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Bibliographic Details
Published in:Methods (San Diego, Calif.) Calif.), 1996-02, Vol.9 (1), p.74-83
Main Authors: Hozier, John C., Hall, Bryan K., Sims, Kenneth R., Liechty, Melissa C., Chen-Liu, Linda, Davis, Lisa M.
Format: Article
Language:English
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Summary:It is now possible to start at a cytogenetically defined position in any eucaryotic genome and proceed toward isolation and identification of candidate genes known to map to that position, taking advantage of the new PCR amplification technology to produce position-specific DNA. The starting material for this very useful exercise is DNA microdissected from standard cytogenetic preparations. Here we describe techniques for acquiring useful samples of such DNA and strategies for the use of chromosome region-specific DNA for gene discovery projects.
ISSN:1046-2023
1095-9130
DOI:10.1006/meth.1996.0010