The relative influence of the topography and chemistry of TiA16V4 surfaces on osteoblastic cell behaviour

Proliferation and adhesion of mouse (MC3T3-E1) osteoblastic cells and primary human osteoblastic cells were carried out on Ti6A14V titanium alloy samples with varied surface roughnesses. Mechanically or manually polished surfaces were prepared to produce respectively non-oriented or oriented residua...

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Bibliographic Details
Published in:Biomaterials 2000-08, Vol.21 (15), p.1567-1577
Main Authors: Anselme, K, Linez, P, Bigerelle, M, Le Maguer, D, Le Maguer, A, Hardouin, P, Hildebrand, H F, Iost, A, Leroy, J M
Format: Article
Language:English
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Summary:Proliferation and adhesion of mouse (MC3T3-E1) osteoblastic cells and primary human osteoblastic cells were carried out on Ti6A14V titanium alloy samples with varied surface roughnesses. Mechanically or manually polished surfaces were prepared to produce respectively non-oriented or oriented residual polishing grooves. Sand-blasted surfaces were prepared using 500 mu m or 3 mm alumina particles. Surface roughness parameters showed a negative correlation in comparison to proliferation and adhesion parameters. X-ray microprobe chemical surface microanalysis showed complete disturbance of the surface element composition of the Ti6A14V alloy following sand-blasting treatment. An AlO sub(x)-enriched layer was observed on sample surfaces. This may lead to the suspicion that the concomittant effect of surface roughness amplitude and AlO sub(x) surface concentration has an effect on osteoblastic cell proliferation and adhesion. These findings show the significance of chemical surface analysis after any surface treatment of titanium-based implants before any biological use.
ISSN:0142-9612