Osmoregulation and the Genetic Induction of Glycerol-3-phosphate Dehydrogenase by NaCl in the Euryhaline Yeast Debaryomyces hansenii

: In the yeasts Saccharomyces cerevisiae and Debaryomyces hansenii, glycerol-3-phosphate dehydrogenase (NAD+-GPD) is believed to be the enzyme controlling the synthesis of glycerol in osmotically challenged cells. Consistent with this concept, immunoblot analyses demonstrated quantitative increases...

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Bibliographic Details
Published in:Marine biotechnology (New York, N.Y.) N.Y.), 1999-05, Vol.1 (3), p.230-238
Main Authors: Thomé, PE, Trench, RK
Format: Article
Language:English
Subjects:
Baking yeast
Brackish
Cells
Debaryomyces hansenii
Dehydrogenase
Dehydrogenases
Enzymes
Euryhalinity
Gene regulation
Glycerol
Glycerol-3-phosphate
Glycerol-3-phosphate dehydrogenase
mRNA
NAD
Nucleic acids
Osmoregulation
Phosphates
Ribonucleic acid
RNA
Saccharomyces cerevisiae
Sodium chloride
Transcription
Yeasts
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Summary:: In the yeasts Saccharomyces cerevisiae and Debaryomyces hansenii, glycerol-3-phosphate dehydrogenase (NAD+-GPD) is believed to be the enzyme controlling the synthesis of glycerol in osmotically challenged cells. Consistent with this concept, immunoblot analyses demonstrated quantitative increases in NAD+-GPD 30 minutes after the addition of NaCl to actively growing D. hansenii cells; Northern blot analyses showed increases in GPD-messenger RNA within 15 minutes after the addition of salt. There appears to be an upper limit of salt concentration above which the cells appear to be less responsive. The results we describe support the concept of transcriptional regulation of NAD+-GPD in D. hansenii.
ISSN:1436-2228
1436-2236
DOI:10.1007/pl00011772