Plant regeneration from callus and suspension cultures of Valeriana edulis ssp. procera via simultaneous organogenesis and somatic embryogenesis

• Published in: Plant science (Limerick) 2000-02, Vol.151 (2), p.115-119
• Main Authors: Castillo, Patricia, Márquez, Judith, Rubluo, Abraham, Hernández, Georgina, Lara, Miguel
• Format: Article
• Language: English
• Subjects: 2,4-D; Biological and medical sciences; Biotechnology; callus; cell suspension culture; culture media; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; germination; histology; In vitro propagation: entire plant regeneration from tissues and cell cultures; kinetin; leaves; Methods. Procedures. Technologies; naphthaleneacetic acid; Organogenesis; Plant cells and fungal cells; plant development; Plant regeneration; regenerative ability; rooting; shoots; Somatic embryogenesis; tissue culture; Valeriana
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/S0168-9452(99)00203-4

Plants of the genus Valeriana produce irioid esters of biotechnological interest, known as valepotriates. The regeneration of Valeriana edulis ssp. procera via indirect organogenesis and somatic embryogenesis from leaf-derived callus and suspension cultures, is presented. For induction of callus, leaves were cultured on semisolid MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin. Embryogenic and organogenic callus development was observed after 16 weeks. Calli were transferred to semisolid and liquid Murashige and Skoog (MS) medium supplemented with kinetin and 1-naphthaleneacetic acid (NAA). Appearance of shoot and somatic embryo occurred 4 weeks later. Shoots were rooted and somatic embryos were germinated and were transferred to the greenhouse. A better organogenic and embryogenic response was observed from suspension cultures. Histological observations of morphogenic callus revealed that both somatic embryos and shoots arose from the same type of callus.