Differences in growth and water relations among Phaseolus vulgaris cultivars in response to induced drought stress

• Published in: Environmental and experimental botany 2000-06, Vol.43 (3), p.227-237
• Main Authors: Costa França, Marcel G, Pham Thi, Anh Thu, Pimentel, Carlos, Pereyra Rossiello, Roberto O, Zuily-Fodil, Yasmine, Laffray, Daniel
• Format: Article
• Language: English
• Subjects: Drought; Growth; Membrane damage; Net assimilation rate; Osmotic potential; Phaseolus vulgaris; Stomatal conductance
• ISSN: 0098-8472; 1873-7307
• DOI: 10.1016/S0098-8472(99)00060-X

Relatively little ecophysiological research has been conducted to determine the responses to drought of Phaseolus vulgaris. Four bean cultivars (cvs.) from Brazil, A320, Carioca, Ouro Negro and Xodó were submitted to an imposed water deficit in order to evaluate the importance of some adaptive mechanisms of drought resistance through the analysis of growth parameters, water status, gas exchange and indicators of tolerance mechanisms at the cellular level. During the drought treatment, relative growth rates were more reduced for A320 and Xodó than Carioca and Ouro Negro. A320 closed its stomata very rapidly and complete stomatal closure was obtained at Ψ w=−0.6 MPa, in contrast to the other cvs. where stomata were fully closed only at Ψ w=−0.9 MPa. Net assimilation rates were closely related to stomatal conductances. Mechanisms at the cellular level appeared to be mostly important for higher tolerance. Carioca and Ouro Negro, when compared to A320 and Xodó, were characterized by having better drought tolerance mechanisms and higher tissue water retention capacity leading to a better growth under water deficits. The leaf dehydration rates of those cvs. were slow whereas those of the drought sensitive cvs. were rapid. The results were confirmed by the electrolyte leakage test and leaf osmotic potential measurements, which indicated higher membrane resistance and osmotic adjustment in the two tolerant cvs. Carioca and Ouro Negro. It appears from this study that despite being cultivated in the same geographical region, the four cvs. of P. vulgaris displayed somewhat different drought adaptive capacities for prolonged drought during the vegetative phase.