Occurrence and Multiple Antibiotic Resistance Profiles of Non-Fermentative Gram-Negative Microflora in Five Brands of Non-Carbonated French Bottled Spring Water

• Published in: Microbial ecology 2000-05, Vol.39 (4), p.322-329
• Main Authors: MARY, P, DEFIVES, C, HORNEZ, J. P
• Format: Article
• Language: English
• Subjects: Antibiotic resistance; Antibiotics; Bacteria; Biological and medical sciences; Bottled water; Burkholderia cepacia; Food industries; Fundamental and applied biological sciences. Psychology; Microorganisms; Mineral water; Non alcoholic beverage industries and mineral waters; Phenotypes; Pseudomonas; Stenotrophomonas maltophilia
• ISSN: 0095-3628; 1432-184X

Five brands of French bottled mineral water were analyzed by heterotrophic plate counts (HPC) and for the presence of multiple antibiotic resistant bacteria. HPC at 22°C were around 104colony forming units ml-1on R2A medium. Enumeration on PCA/10, MH, and especially PCA and King B media was less efficient. At 37°C, HPC were two to three orders of magnitude less than at 22°C. Moreover, phenotypic diversity (7 to 15 phenotypes) was optimal on R2A incubated at 22°C. All isolates were identified as non-fermentative Gram-negative rods and 75% were non-identifiable with the API 20NE system. Stenotrophomonas maltophilia and fluorescent Pseudomonas were isolated on VIA and CFC selective agar media, respectively. Burkholderia cepacia strains were not isolated on BCSA medium. The species S. maltophilia was found in 33%, 28%, and 11% of sample from springs A, D, and E, respectively. Independent of brand, isolates from HPC media were less efficient to achieve confluent growth in 18 h on MH at 30 or 37°C (0 to 40%) than isolates from selective media (28 to 63%). Seventy percent of the total isolates from dominant microflora ($1-5\times 10^{3}$CFU ml-1on HPC media) were resistant against two or four antibiotics. The antibiotics concerned were principally aztreonam, ampicillin, and nalidixic acid. The remaining dominant bacteria showed a 6-9 multiple antibiotic resistant (MAR) pattern. All isolates were susceptible to newer antimicrobial agents. Owing to their low nutrient and temperature requirements, these isolates are unlikely to cause concern to public heath. Fifty percent of strains isolated from selective media (non-dominant microflora, 4-40 CFU l-1) showed a 10-18 MAR pattern and 33%, identified as S. maltophilia, a 20-27 MAR pattern. However, minocycline was effective against all isolates. Owing to its low concentration, colonization of human intestine by MAR S. maltophilia is unlikely.