Identification and characterization of the trnS/pseudo-tRNA/ nad3/ rps12 gene cluster from Coix lacryma- jobi L: organization, transcription and RNA editing

During a study of mitochondrial sequence conservation between the liverwort Marchantia polymorpha and several Angiosperm species, as revealed by heterologous hybridization experiments, the trnS/pseudo-tRNA/ nad3/ rps12 gene cluster in Coix lacryma-jobi L., an Asian grass species from the Andropogone...

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Bibliographic Details
Published in:Plant science (Limerick) 2000-09, Vol.158 (1), p.97-105
Main Authors: Dias, Sandra Martha G., Siqueira, Susely F., Lejeune, Bernard, de Souza, Anete P.
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Classical and quantitative genetics. Population genetics. Molecular genetics
Coix lacryma- jobi L
Coix lacryma-jobi
Fundamental and applied biological sciences. Psychology
Generalities. Genetics. Plant material
Genes. Genome
Genetics and breeding of economic plants
itrnS gene
Marchantia polymorpha
Mitochondrial DNA
Molecular and cellular biology
Molecular genetics
nad3 gene
NADH dehydrogenase subunit 3
orf167 gene
pseudo-tRNA gene
Ribosomal S12
RNA editing
rps12 gene
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Summary:During a study of mitochondrial sequence conservation between the liverwort Marchantia polymorpha and several Angiosperm species, as revealed by heterologous hybridization experiments, the trnS/pseudo-tRNA/ nad3/ rps12 gene cluster in Coix lacryma-jobi L., an Asian grass species from the Andropogoneae, was identified using the mitochondrial probe orf167 from M. polymorpha. The Coix gene cluster was cloned and sequenced, and its expression analyzed. The gene sequence and gene locus organization were found to be similar to the corresponding cluster in wheat and maize. Northern hybridization and reverse transcription-polymerase chain reaction analyses indicated that nad3 and rps12 genes were co-transcribed as a 1.25 kb RNA molecule. The transcript displayed 20 and six RNA edition sites, in the nad3 and rps12 genes, respectively, that changed the codon identities to amino acids, which are better conserved in different organisms. Twenty-three cDNA clones were analysed for the edition process and revealed different partial editing patterns without apparent sequential processing.
ISSN:0168-9452
1873-2259
DOI:10.1016/S0168-9452(00)00308-3